NUCLEAR AND AXONAL LOCALIZATION OF CA2+ CALMODULIN-DEPENDENT PROTEIN-KINASE TYPE-GR IN RAT CEREBELLAR CORTEX
NUCLEAR AND AXONAL LOCALIZATION OF CA2+ CALMODULIN-DEPENDENT PROTEIN-KINASE TYPE-GR IN RAT CEREBELLAR CORTEX
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DOI：
10.1073/pnas.88.7.2850
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发表时间：
1991-04-01
影响因子：
11.1
通讯作者：
SAHYOUN, N
中科院分区：
文献类型：
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作者：
JENSEN, KF;OHMSTEDE, CA;SAHYOUN, N
颗粒细胞富集钙/钙调素依赖性蛋白激酶（CaM kinase-Gr）是近年来发现的一种神经元特异性酶。该激酶强烈磷酸化突触蛋白I，并含有多聚谷氨酸序列，这表明它也与染色质有关。一个可能的作用，突触蛋白I磷酸化和核钙离子信号的钙调素激酶Gr分布的免疫化学和超微结构检查的支持。CaM激酶-Gr免疫反应性存在于大鼠小脑的分子和颗粒细胞层。这种模式对应于颗粒细胞轴突和细胞核中的酶的发生。免疫印迹证实了这些发现。因此，钙调素激酶Gr可以介导和协调不同的亚细胞区室内的Ca 2+信号。
The granule cell-enriched Ca2+/calmodulin-dependent protein kinase (CaM kinase-Gr) is a recently discovered neuron-specific enzyme. The kinase avidly phosphorylates synapsin I and contains a polyglutamate sequence, which suggests an association with chromatin as well. A possible role in synapsin I phosphorylation and in nuclear Ca2+ signaling was supported by immunochemical and ultrastructural examination of CaM kinase-Gr distribution. CaM kinase-Gr immunoreactivity was present in the molecular and granule cell layers of the rat cerebellum. This pattern corresponded to the occurrence of the enzyme in the granule cell axons and nuclei, respectively. Immunoblots confirmed these findings. Thus, CaM kinase-Gr may mediate and coordinate Ca2+-signaling within different subcellular compartments.