Packaging cordycepin phycocyanin micelles for the inhibition of brain cancer
Packaging cordycepin phycocyanin micelles for the inhibition of brain cancer
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包装虫草素藻蓝蛋白胶束抑制脑癌
DOI：
10.1039/c7tb00994a
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发表时间：
2017
影响因子：
7
通讯作者：
Guan Yan-Qing
中科院分区：
文献类型：
--
作者：
Zhao Mengyang;Chen Liyi;Chen Wuya;Meng Zhan;Hu Kaikai;Du Shiwei;Zhang Lingkun;Yin Liang;Wu Baoyan;Guan Yan-Qing
虫草素作为一种天然抗癌药物已被成功应用，但其在体内代谢迅速。因此，纳米胶囊是一种很有前途的提高其生物利用度的方法。在本研究中，我们采用绿色合成工艺来制备新型自组装的藻蓝蛋白-葡聚糖-虫草素(Phy-Dex-Cord)胶束，用于高效地包裹和传递虫草素。我们首次使用美拉德反应方法将葡聚糖接枝到藻蓝蛋白上，形成了藻蓝蛋白-葡聚糖复合体。虫草素通过自组装形成藻蓝蛋白-葡聚糖复合体，形成胶束。其物理化学性质和表征结果表明，Phy-Dex-Cord胶束呈球形，粒径分布均匀，约为60 nm。此外，体外和体内的抗癌活性表明，Phy-Dex-Cord胶束对C6细胞的抑制作用与游离虫草素和游离藻蓝蛋白相当，甚至更强，且没有副作用。
Cordycepin has been successfully used as a natural anti-cancer drug, but it is rapidly metabolized in vivo. Nanoencapsulation is thus a promising method to improve its bioavailability. In this study, we adopted a green synthesis process to develop novel self-assembling phycocyanin–dextran–cordycepin (Phy–Dex–Cord) micelles for efficient cordycepin encapsulation and delivery. We first used the Maillard reaction method to graft dextran onto phycocyanin, forming a phycocyanin–dextran complex. Through the self-assembly of the cordycepin parcel to the phycocyanin–dextran complex, the micelles were formed. Their physical and chemical properties and characterization results showed that Phy–Dex–Cord micelles have a spherical shape and consistent size distribution of about 60 nm. In addition, anti-cancer activities in vitro and in vivo revealed that the Phy–Dex–Cord micelles have a comparable or even stronger inhibitory effect against C6 cells than do free cordycepin and free phycocyanin and no side effects.