Construction of mES Derived 3D-Pacemaker Tissues by Layer-by-Layer Nanofilm Coating
Construction of mES Derived 3D-Pacemaker Tissues by Layer-by-Layer Nanofilm Coating
复制标题
通过逐层纳米膜涂层构建 mES 衍生的 3D 起搏器组织
DOI：
10.1002/cnma.201600031
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
M. Akashi
中科院分区：
文献类型：
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作者：
Y. Amano;T. Igarashi;A. Nishiguchi;M. Matsusaki;Y. Saito;K. Nakamura;H. Ito;M. Akashi
对于心律失常的治疗，通常采用电子起搏器。然而，它们存在生物不相容性和电池限制等问题。最近，使用表达超极化激活的环核苷酸门控4（HCN 4）通道的细胞作为起搏细胞而不是电子起搏器引起了越来越多的关注。然而，由于移植细胞的低植入率和炎症反应的风险，细胞移植治疗不够有效。为了克服这些问题，我们构建了由小鼠胚胎细胞来源的心肌细胞（mESC-CMs）组成的3D起搏器组织，其中HCN 4基因已通过细胞累积技术导入其中。所获得的组织比对照组织搏动更快，并且每分钟搏动（BPM）随着组织厚度明显增加。这是第一份报告提出BPM和组织厚度之间的关系。此外，起搏器组织可以控制修补组织的跳动。
For the treatment of cardiac arrhythmia, electronic pacemakers are often employed. However, they have issues such as bio‐incompatibility and battery limitations. Recently, the use of cells expressing hyperpolarization‐activated cyclic nucleotide‐gated 4 (HCN4) channels for use as pacemaker cells instead of electronic pacemakers has attracted increasing attention. However, the cell transplantation treatment was not sufficiently effective because of the low engraftment rate of the transplanted cells and the risk of inflammatory reactions. Here, in order to overcome these issues, we constructed 3D‐pacemaker tissues composed of mouse‐embryonic‐cell‐derived cardiomyocytes (mESC‐CMs) in which the HCN4 gene had been introduced by the cell accumulation technique. The obtained tissues beat faster than control tissues and beats per minute (BPM) increased clearly with tissue thickness. This is the first report suggesting the relation between BPM and tissue thickness. Moreover, the pacemaker tissue could control the beating of the patched tissue.