Influenza A virus infection of human Schwann cells in vitro.
Influenza A virus infection of human Schwann cells in vitro.
复制标题
甲型流感病毒体外感染人雪旺细胞。
DOI：
10.1080/0036554021000028092
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发表时间：
2003
影响因子：
1.4
通讯作者：
Fregien,Nevis
中科院分区：
文献类型：
--
作者：
Levine,Joshua;Buchman,CraigA;Fregien,Nevis
目的——突发性感音神经性听力损失、前庭神经炎、声带麻痹和贝尔麻痹与病毒病因有关，原因是神经细胞感染。本研究的目的是确定雪旺细胞是否能支持人类甲型流感病毒的感染，从而代表病毒与宿主相互作用的一个合理的替代位点。病毒感染雪旺细胞可导致炎症介质分泌、白细胞募集、脱髓鞘和神经损伤。材料和方法——将培养的人类雪旺细胞暴露于人类甲型流感病毒中。接种后不同时间（0、24、48和72 h），采用光镜、免疫细胞化学和甲型流感病毒特异性逆转录酶聚合酶链反应（RT-PCR）检测感染情况。一组未暴露的细胞作为对照。结果：暴露于病毒后，早在接种后24小时就可以看到空泡化、细胞扩增和脱离培养皿。暴露的细胞在24、48和72 h时显示出甲型流感病毒抗原的免疫细胞化学染色阳性。利用RT-PCR检测到甲型流感病毒特异性mRNA的急剧上升。结论：人雪旺细胞可感染甲型流感病毒。进一步的研究将评估该模型中的炎症反应。
Objectives--Sudden sensorineural hearing loss, vestibular neuronitis, vocal fold paralysis and Bell's palsy have been associated with a viral etiology, due to the infection of nerve cells. The goal of this research was to ascertain whether Schwann cells can support infection with human influenza A virus and thereby represent a plausible alternative site for virus-host interaction. Viral infection of Schwann cells may lead to secretion of inflammatory mediators, leukocyte recruitment, demyelination and nerve damage.Material and methods--Cultured human Schwann cells were exposed to human influenza A virus. Infection was assayed at various times post-inoculation (0, 24, 48 and 72 h) using light microscopy, immunocytochemistry and influenza A virus-specific reverse transcriptase polymerase chain reaction (RT-PCR). A group of unexposed cells served as controls.Results--Following exposure to the virus, vacuolization, cellular expansion and detachment from the dish were seen as early as 24 h post-inoculation. The exposed cells demonstrated positive immunocytochemical staining for influenza A virus antigen at 24, 48 and 72 h. Using RT-PCR, a sharp rise in influenza A virus-specific mRNA was detected.Conclusions--Human Schwann cells can be infected with human influenza A virus. Further studies will assess the inflammatory response in this model.