A freeze-fracture study of the periodontal ligament of the rat.
A freeze-fracture study of the periodontal ligament of the rat.
复制标题
大鼠牙周膜冷冻断裂研究。
DOI：
10.1111/j.1600-0765.1983.tb00359.x
复制
发表时间：
1983
影响因子：
3.5
通讯作者：
Garant,PR
中科院分区：
文献类型：
--
作者：
Cho,MI;Garant,PR
用冷冻断裂法对大鼠牙周膜成纤维细胞进行了原位研究。在超微结构水平上可以看到细胞膜的原生质面和胞外面以及粗面内质网、高尔基体和其他细胞质细胞器的大表面。显示了代表膜蛋白的膜内颗粒，并测量了它们在不同膜中的浓度。成纤维细胞表面呈现为粗糙斑块的特征被解释为代表可能的细胞与基质相互作用的区域。讨论了冷冻断裂技术在PDL成纤维细胞-基质接触的进一步研究中的应用。
Fibroblasts of the rat periodontal ligament were studiedin situby the freeze‐fracture technique. Large surfaces of the protoplasmic and extracellular faces of the cell membrane as well as, the membranes of the RER, Golgi apparatus, and other cytoplasmic organelles were viewed at the ultrastructural level. Intramembrane particles representing membrane proteins were visualized, and their concentration in various membranes measured.Features of the fibroblast cell surface appearing as rough patches were interpreted to represent areas of possible cell‐to‐matrix interaction. The use of the freeze‐fracture technique in further studies of PDL fibroblast‐matrix contact is discussed.