Insertion site preferences of the P transposable element in Drosophila melanogaster.
Insertion site preferences of the P transposable element in Drosophila melanogaster.
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DOI：
10.1073/pnas.97.7.3347
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发表时间：
2000-03
影响因子：
11.1
通讯作者：
Liao Gc;E. J. Rehm;Gerald M. Rubin
中科院分区：
文献类型：
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作者：
Liao Gc;E. J. Rehm;Gerald M. Rubin
我们确定了2266个未筛选的P元件插入事件中插入位点的基因组序列。对这些插入位点的基因组DNA的物理特性（如碱基组成、可弯曲性、亲A性、蛋白质诱导的可变形性以及B - DNA扭转）进行评估后发现，它们与染色体平均DNA存在显著差异。通过检查大沟中的潜在氢键位点，我们确定了一种以P元件插入所产生的8bp靶位点重复序列为中心的14bp回文模式。我们的研究结果表明，P元件转座机制具有二重旋转对称，并识别插入位点的一种结构特征，而非特定的序列基序。
We determined the genomic sequence at the site of insertion in 2,266 unselected P element insertion events. Estimating physical properties of the genomic DNA at these insertion sites-such as base composition, bendability, A-philicity, protein-induced deformability, and B-DNA twist-revealed that they differ significantly from average chromosomal DNA. By examining potential hydrogen bonding sites in the major groove, we identified a 14-bp palindromic pattern centered on the 8-bp target site duplication that is generated by P element insertion. Our results suggest that the P-element transposition mechanism has a two-fold dyad symmetry and recognizes a structural feature at insertion sites, rather than a specific sequence motif.