Role of cytochrome P450 genes of Trichoderma atroviride T23 on the resistance and degradation of dichlorvos
Role of cytochrome P450 genes of Trichoderma atroviride T23 on the resistance and degradation of dichlorvos
复制标题
深绿木霉T23细胞色素P450基因对敌敌畏的抗性和降解作用
DOI：
10.1016/j.chemosphere.2021.133173
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发表时间：
2021
期刊：
影响因子：
8.8
通讯作者：
Jie Chen
中科院分区：
文献类型：
--
作者：
Jianan Sun;Valliappan Karuppiah;Yaqian Li;Sivakumar P;ian;Subramanian Kumaran;Jie Chen
木霉菌已被广泛应用于外源物质的降解。本研究中，39个细胞色素P450基因的开放阅读框从T。克隆了深绿素T23基因组，发现其分布于29个科，分属21个分支。其中，21个细胞色素P450基因参与了外源物的降解。在敌敌畏浓度为100 μg/mL、300 μg/mL、500 μ g/mL和1000 μg/mL时，P450基因在24 h的定量表达呈现7种不同的表达模式。TaCyp 548、TaCyp 620、TaCyp 52、TaCyp 528、TaCyp 504家族的P450基因的相对表达与对照相比上调至少1倍。值得注意的是，TaCyp 548 -2的缺失降低了2，2-二氯乙醇的浓度。此外，观察到属于ω-羟化酶家族的TaCyp 548 -2负责脂肪酸氧化和乙酸、丙酸、异丁酸和二丁酸的产生以将2，2_二氯乙醇转化为2，2_二氯乙醇乙酸酯。本研究证明了木霉P450基因参与敌敌畏的降解，作为一种环境重要的生物控制剂，可持续农业。
The Trichoderma has been extensively used to degrade the xenobiotics. In the present study, thirty-nine open reading frames of cytochrome P450 genes from T. atroviride T23 genome was cloned and it was found to be distributed in 29 families under 21 clades. Among them, 21 cytochrome P450 genes were involved in the degradation of xenobiotics. The quantitative expression of P450 genes in the presence of dichlorvos at 24 h showed 7 different expression patterns in the presence of 100 μg/mL, 300 μg/mL, 500 μg/mL and 1000 μg/mL of dichlorvos. The relative expression of P450 genes belongs to the family of TaCyp548, TaCyp620, TaCyp52, TaCyp528, TaCyp504 were upregulated at least 1-fold compared to the control. Significantly, the deletion of TaCyp548-2 reduced the concentration of 2,2-dichloroethanol. Further, it was observed that TaCyp548-2 belongs to the ω-hydroxylase family was responsible for fatty acid oxidation and the production of acetic acid, propionic acid, isobutyric acid and dibutyric acid to convert the 2,2-dichloroethanol to 2,2-dichloroethanolacetate. This study evidenced the involvement of Trichoderma P450 genes on dichlorvos degradation as an environmentally significant Biological control agent for the sustainable agriculture.