Estimate of the genomic mutation rate deleterious to overall fitness in E-coli
Estimate of the genomic mutation rate deleterious to overall fitness in E-coli
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DOI：
10.1038/381694a0
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发表时间：
1996-06-20
期刊：
影响因子：
64.8
通讯作者：
Lynch, M
中科院分区：
文献类型：
--
作者：
Kibota, TT;Lynch, M
突变是一把双刃剑：它们是进化所依赖的遗传变异的最终来源，但大多数影响适应性(生存能力和繁殖成功)的突变似乎是有害的(1)。影响小的有害突变可以逃脱自然选择，应该在小种群中积累(2-4个)。有害突变积累带来的适合度降低可能在性别(5-7)、配偶选择(8，9)和二倍体生命周期(10)的进化以及小种群(11，12)的灭绝中具有重要意义。然而，几乎没有经验数据。对果蝇生存能力的基因组有害突变率的最低估计令人惊讶地高(1，13，14)，导致推测总适应率可能超过每代每个个体1.0个突变(5，6)。在这里，我们使用大肠杆菌来提供对微生物总适应度的基因组有害突变率的估计。我们估计，每种微生物的有害突变比率超过0.0002。
MUTATIONS are a double-edged sword: they are the ultimate source of genetic variation upon which evolution depends, yet most mutations affecting fitness (viability and reproductive success) appear to be harmful(1). Deleterious mutations of small effect can escape natural selection, and should accumulate in small populations(2-4). Reduced fitness from deleterious-mutation accumulation may be important in the evolution of sex(5-7), mate choice(8,9), and diploid life-cycles(10), and in the extinction of small populations(11,12). Few empirical data exist, however. Minimum estimates of the genomic deleterious-mutation rate for viability in Drosophila melanogaster are surprisingly high(1,13,14), leading to the conjecture that the rate for total fitness could exceed 1.0 mutation per individual per generation(5,6). Here we use Escherichia coli to provide an estimate of the genomic deleterious-mutation rate for total fitness in a microbe. We estimate that the per-microbe rate of deleterious mutations is in excess of 0.0002.