Interaction and Effects of Bacteria Addition on Dichlorodiphenyltrichloroethane Biodegradation by Daedalea dickinsii
Interaction and Effects of Bacteria Addition on Dichlorodiphenyltrichloroethane Biodegradation by Daedalea dickinsii
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DOI：
10.1007/s00284-020-02305-8
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发表时间：
2021-01
影响因子：
2.6
通讯作者：
H. D. Rizqi;Adi Setyo Purnomo;Ichiro Kamei
中科院分区：
文献类型：
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作者：
H. D. Rizqi;Adi Setyo Purnomo;Ichiro Kamei
有机氯农药残留已成为环境污染的主要问题。二氯二苯三氯乙烷（DDT）是最常见的持久性有机氯农药之一，继续对人类健康和环境构成严重威胁。已经开发了一些处理方法来减少和最大限度地减少使用滴滴涕的不利影响，包括用褐腐真菌（BRF）进行生物降解。但是，BRF降解DDT的降解率仍然很低，需要较长的孵育时间。因此，需要提高BRF降解DDT的能力。研究了添加细菌对黄腐菌Daedalea dickinsii降解DDT的影响及其相互作用。对D.结果表明，添加铜绿假单胞菌对狄氏霉的生长没有抑制作用。dickinsii。同时，添加枯草芽孢杆菌对D. dickinsii。加入10 ml（1 ml = 1.05 × 109 CFU/ml菌细胞）P.奥克拉荷马湾枯草杆菌能促进D. dickinsii分别从53.61%提高到96.70%和67.60%。当添加10 ml磷时，DDT的降解能力最高。从空中进入D.迪金森氏养殖，其中混合培养物产生1，1-二氯-2，2-双（4-氯苯基）乙烷（DDD）和1-氯-2，2-双（4-氯苯基）乙烯（DDMU）的最终代谢物。本研究表明，P.好氧微生物可用于优化D. dickinsii。
The residue of organochlorine pesticides (OCPs) has been a major pollution problem in our environment. Dichlorodiphenyltrichloroethane (DDT) is one of the most common persistent OCPs that continue to pose a serious risk to human health and the environment. Some treatment methods have been developed to reduce and minimize the adverse impacts of the use of DDT, including biodegradation with brown-rot fungi (BRF). However, DDT degradation using BRF has still low degradation rate and needs a long incubation time. Therefore, the ability of BRF need to be enhanced to degrade DDT. Interaction and effect of bacteria addition on biodegradation of DDT by brown-rot fungusDaedalea dickinsiiwere investigated. The interaction assay betweenD. dickinsiiwith bacteria addition showed that the addition of bacteriumPseudomonas aeruginosadid not provide resistance to the growth ofD. dickinsii. Meanwhile, bacteriumBacillus subtilisaddition has an inhibitory effect on the growth ofD. dickinsii. The addition of 10 ml (1 ml = 1.05 × 109CFU/ml bacteria cell) ofP. aeruginosaandB. subtiliswas able to improve DDT biodegradation byD. dickinsiifrom 53.61% to 96.70% and 67.60%, respectively. The highest biodegradation capability of DDT was obtained through addition of 10 ml ofP. aeruginosainto theD. dickinsiiculture in which the mixed cultures produce final metabolites of 1,1-dichloro-2,2-bis(4-chlorophenyl)ethane (DDD) and 1-chloro-2,2-bis(4-chlorophenyl)ethylene (DDMU). This study indicated that the addition ofP. aeruginosacan be used for optimization of DDT biodegradation byD. dickinsii.