Synthetic antibody technologies
Synthetic antibody technologies
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DOI：
10.1016/j.sbi.2013.11.003
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发表时间：
2014-02-01
影响因子：
6.8
通讯作者：
Sidhu, Sachdev S.
中科院分区：
文献类型：
--
作者：
Adams, Jarrett J.;Sidhu, Sachdev S.
合成抗体技术能够通过体外选择快速生产亲和试剂。合成抗体的产生依赖于复杂的设计策略来产生编码针对分子识别优化的抗体群体的组合多样性文库。该技术利用展示技术，使体外扩增，选择和操纵抗体。这些方法的快速但高度受控的性质为基础和临床研究开辟了新的途径。在这里，我们回顾了结构生物学的最新进展，促进了合成抗体，以及在库设计和选择方法的进展。
Synthetic antibody technologies enable the rapid production of affinity reagents through in vitro selections. The production of synthetic antibodies relies on sophisticated design strategies to produce combinatorial diversity libraries that encode antibody populations optimized for molecular recognition. The technology takes advantage of display technologies that enable amplification, selection and manipulation of antibodies in vitro. The rapid yet highly controlled nature of these methods has opened new avenues in basic and clinical research. Here we review recent advances in structural biology facilitated by synthetic antibodies, as well as advances in library designs and selection methods.