Circadian rhythm disorder of thrombosis and thrombolysis-related gene expression in apolipoprotein E knock-out mice.
Circadian rhythm disorder of thrombosis and thrombolysis-related gene expression in apolipoprotein E knock-out mice.
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DOI：
10.3892/ijmm_00000002
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发表时间：
2008
影响因子：
5.4
通讯作者：
Xueqing Zhang;F. Zhao;Chen Xu;Chao Lu;Huiming Jin;Sifen chen;R. Qian
中科院分区：
文献类型：
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作者：
Xueqing Zhang;F. Zhao;Chen Xu;Chao Lu;Huiming Jin;Sifen chen;R. Qian
斑块破裂和随后的栓塞以及血栓形成是继发于动脉粥样硬化的急性心肌梗死和卒中的主要原因。派-1、t-PA、TF和ET-1是血栓形成和血栓溶解的相关因子，在血栓形成和斑块破裂中起重要作用。由于急性心肌梗死和中风更容易发生在早上6点和下午12点之间，而不是在一天中的其他时间，我们研究了昼夜节律与正常和动脉粥样硬化小鼠的派-1，t-PA，TF和ET-1之间的关系。动脉粥样硬化是在apoE-/-小鼠喂养正常饮食或高胆固醇饮食。采用实时荧光定量RT-PCR方法检测不同Zeitgeber时间（ZT 0、ZT 4、ZT 8、ZT 10、ZT 12、ZT 14、ZT 16、ZT 20）时对照C57 BL/6 J小鼠和动脉粥样硬化小鼠心脏派-1、t-PA、TF和ET-1的表达。派-1、t-PA、TF和ET-1在C57 BL/6 J小鼠中的表达在ZT 14和ZT 16之间达到高峰，在ZT 10达到低谷。它们在apoE-/-小鼠中的表达失去了昼夜节律。它们在喂食高胆固醇饮食的apoE-/-小鼠中的表达在ZT 4达到峰值，表明昼夜节律相反。提示派-1、t-PA、TF和ET-1表达的昼夜变化可能参与了心肌梗死和脑卒中的发生。
Plaque rupture and subsequent embolism as well as thrombosis are major causes of acute myocardial infarction and stroke secondary to atherosclerosis. Pai-1, t-PA, TF and ET-1 are thrombosis- and thrombolysis-related factors which play important roles in thrombosis formation and plaque rupture. Since acute myocardial infarction and stroke are more likely to occur between 6 a.m. and 12 p.m. than at another time of the day, we studied the relationship between circadian rhythm and Pai-1, t-PA, TF and ET-1 in normal and atherosclerotic mice. Atherosclerosis was developed in apoE-/- mice fed a normal diet or a high cholesterol diet. The expression of Pai-1, t-PA, TF and ET-1 in the hearts of control C57BL/6J mice and atherosclerotic mice was measured by real-time RT-PCR at different Zeitgeber times (ZT) including ZT0, ZT4, ZT8, ZT10, ZT12, ZT14, ZT16 and ZT20. The expression of Pai-1, t-PA, TF and ET-1 peaked between ZT14 and ZT16 and bottomed at ZT10 in C57BL/6J mice. Their expression in apoE-/- mice fed a normal diet lost circadian rhythm. Their expression in apoE-/- mice fed a high cholesterol diet peaked at ZT4, indicating a reverse circadian rhythm. Our result indicates that circadian changes in the expression of Pai-1, t-PA, TF and ET-1 may be involved in the onset of myocardial infarction and stroke.