Expression of extracellular matrix metalloproteinase inducer (EMMPRIN) in healthy and inflamed human gingival.
Expression of extracellular matrix metalloproteinase inducer (EMMPRIN) in healthy and inflamed human gingival.
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DOI：
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发表时间：
2009-09
影响因子：
1.9
通讯作者：
J. Xiang;Z. Cao;W. Dong;Chengzhang Li
中科院分区：
文献类型：
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作者：
J. Xiang;Z. Cao;W. Dong;Chengzhang Li
目的探讨细胞外基质金属蛋白酶诱导因子（EMMPRIN）在健康牙龈和炎症牙龈中的表达。方法与材料收集9例健康人牙周组织标本和21例慢性牙周炎患者牙周组织标本。采用免疫组化和Western blot方法检测EMMPRIN蛋白的表达。结果EMMPRIN在健康牙龈组织中主要定位于角质形成细胞，在炎症牙龈组织中，EMMPRIN蛋白表达于角质形成细胞、成纤维细胞和炎症细胞。此外，EMMPRIN在炎症牙龈中的表达水平与健康组织相比显著增加（P <0.05）。免疫印迹分析显示，EMMPRIN蛋白在健康牙龈和炎症牙龈中均有表达，且炎症牙龈中EMMPRIN蛋白表达水平显著高于健康对照组（P <0.05）。结论EMMPRIN可能参与牙周组织的生理和病理状态。
OBJECTIVE To evaluate the expression of extracellular matrix metalloproteinase inducer (EMMPRIN) in healthy and inflamed human gingiva. METHOD AND MATERIALS Tissue samples from 9 healthy specimens and 21 specimens with chronic periodontitis were collected. The expression of EMMPRIN protein was detected with immunohistochemical staining and Western blot analysis. RESULTS EMMPRIN was mainly localized in keratinocytes of healthy human gingival tissues; in inflamed gingival tissues, EMMPRIN protein could be detected in keratinocytes, fibroblasts, and inflammatory cells. In addition, EMMPRIN expression level in the inflamed gingiva was increased dramatically compared to that in healthy tissue (P < .05). Using Western blot analysis, EMMPRIN protein was also found to express in both healthy and inflamed gingiva, and the level of EMMPRIN in inflamed gingiva was significantly higher than that in healthy control subjects (P < .05). CONCLUSION EMMPRIN might be involved in the physiologic and pathologic states of periodontal tissues.