IL-15 promotes cytokine production of human T helper cells.
IL-15 promotes cytokine production of human T helper cells.
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IL-15 促进人类 T 辅助细胞的细胞因子产生。
DOI：
10.4049/jimmunol.156.7.2400
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发表时间：
1996
影响因子：
4.4
通讯作者：
H. Okudaira
中科院分区：
文献类型：
--
作者：
A. Mori;M. Suko;O. Kaminuma;S. Inoue;T. Ohmura;Y. Nishizaki;T. Nagahori;Y. Asakura;A. Hoshino;Y. Okumura;G. Sato;K. Ito;H. Okudaira
IL-15是一种新发现的细胞因子，具有与IL-2相似的T细胞和B细胞生长因子活性。在这项研究中，IL-15的一个新的生物学功能，促进细胞因子的产生人Th细胞已被阐明。粉尘螨11（屋尘螨的主要过敏原）特异性人T细胞克隆响应于重组人IL-15以及抗CD 3或IL-2刺激而产生IL-5。IL-5 mRNA在IL-15刺激后3 h可检测到，并在9 h达到最大值。转染到T细胞克隆中的人IL-5启动子/增强子-荧光素酶基因构建体在应答IL-15时被清楚地转录，表明编码区5'上游约500-bp的人IL-5启动子/增强子区段充分应答IL-15。IL-15诱导的IL-5合成被酪氨酸激酶抑制剂除莠霉素A完全抑制，表明酪氨酸激酶参与导致IL-5合成以及IL-15诱导的T细胞增殖的信号转导。尽管通过向培养物中加入抗IL-2中和抗体消除了人外周T细胞的IL-5产生，但IL-15恢复了IL-5合成，尽管有效的IL-2中和。在变应性炎症部位产生的IL-15可能通过诱导T细胞产生IL-5（Th 2细胞因子）在嗜酸性粒细胞的募集和活化中起作用。
IL-15 is a newly identified cytokine that has T cell and B cell growth factor activity similar to that of IL-2. In this study, a novel biologic function of IL-15 to promote cytokine production by human Th cells has been elucidated. Dermatophagoides farinae 11 (a major allergen of house dust mite)-specific human T cell clones produced IL-5 in response to recombinant human IL-15 as well as to either anti-CD3 or IL-2 stimulation. IL-5 mRNA became detectable 3 h after IL-15 stimulation and reached a maximum at 9 h. Human IL-5 promoter/enhancer-luciferase gene construct transfected to T cell clones was clearly transcribed in response to IL-15, indicating that the approximately 500-bp human IL-5 promoter/enhancer segment 5' upstream of the coding region sufficiently responded to IL-15. IL-15-induced IL-5 synthesis was completely inhibited by the tyrosine kinase inhibitor, herbimycin A, suggesting the involvement of tyrosine kinases in the signal transduction leading to IL-5 synthesis as well as to proliferation of T cells induced by IL-15. Whereas IL-5 production by human peripheral T cells was abolished by the addition of anti-IL-2-neutralizing Abs into the culture, IL-15 restored the IL-5 synthesis despite effective IL-2 neutralization. IL-15 produced at the site of allergic inflammation may play a role in the recruitment and activation of eosinophils by inducing IL-5 (a Th2 cytokine) production by T cells.