Presence of two tumor necrosis factor (tnf)-α homologs on different chromosomes of zebrafish (Danio rerio) and medaka (Oryzias latipes)
Presence of two tumor necrosis factor (tnf)-α homologs on different chromosomes of zebrafish (Danio rerio) and medaka (Oryzias latipes)
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DOI：
10.1016/j.margen.2013.10.004
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发表时间：
2014-02-01
期刊：
影响因子：
1.9
通讯作者：
Sakai, Masahiro
中科院分区：
文献类型：
--
作者：
Kinoshita, Shunsuke;Biswas, Gouranga;Sakai, Masahiro
据报道，硬骨鱼中存在多种细胞因子的两种或多种亚型，包括肿瘤坏死因子（tnfs）。尽管斑马鱼 (Danio rerio) 和青鳉鱼 (Oryzias latipes) 拥有两个 trif-alpha 基因，但它们的基因组位置和存在仍有待描述和证实。因此，我们对两种鱼的trif-α和tnf-n与人TNF/淋巴毒素位点进行了二氧化硅鉴定、同线性分析以及它们在斑马鱼中的表达分析。我们从斑马鱼和青鳉中鉴定出 tnf-alpha 的两个同源物（命名为 tnf-alpha 1 和 tnf-alpha 2）和一个 tnf-n 基因。发现这些基因的基因组位置为：tnf-alpha 1、tnf-n和tnf-alpha 2基因分别位于斑马鱼19号和15号染色体以及青鳉11号和16号染色体上。 TNF 家族特征的存在、TNF 基因座与人类染色体的基因保守性、系统发育聚类以及与其他硬骨鱼 TNF 的氨基酸相似性等几个特征证实了它们作为 tnf-alpha 和 tnf-n 的身份。 20 μg mL(-1) LPs体外诱导的斑马鱼头肾细胞中，3个基因在不同组织中均有组成型表达，tnf-α 1和-α 2表达增加，tnf-n配体表达不同。我们的结果表明斑马鱼和青鳉鱼的不同染色体上存在两种 TNF-α 同源物，并将这种由鱼类全基因组复制事件引起的发生率关联起来。 (C) 2013 Elsevier B.V. 保留所有权利。
Two or more isoforms of several cytokines including tumor necrosis factors (tnfs) have been reported from teleost fish. Although zebrafish (Danio rerio) and medaka (Oryzias latipes) possess two trif-alpha genes, their genomic location and existence are yet to be described and confirmed. Therefore, we conducted in silica identification, synteny analysis of trif-alpha and tnf-n from both the fish with that of human TNF/lymphotoxin loci and their expression analysis in zebrafish. We identified two homologs of tnf-alpha (named as tnf-alpha 1 and tnf-alpha 2) and a tnf-n gene from zebrafish and medaka. Genomic location of these genes was found to be as: tnf-alpha 1, and tnf-n and tnf-alpha 2 genes on zebrafish chromosome 19 and 15 and medaka chromosome 11 and 16, respectively. Several features such as existence of TNF family signature, conservation of genes in TNF loci with human chromosome, phylogenetic clustering and amino acid similarity with other teleost TNFs confirmed their identity as tnf-alpha and tnf-n. There were a constitutive expression of all three genes in different tissues, and an increased expression of tnf-alpha 1 and -alpha 2 and a varied expression of tnf-n ligand in zebrafish head kidney cells induced with 20 mu g mL(-1) LPs in vitro. Our results suggest the presence of two tnf-alpha homologs on different chromosomes of zebrafish and medaka and correlate this incidence arising from the fish whole genome duplication event. (C) 2013 Elsevier B.V. All rights reserved.