MEK1 and AKT2 Mutations in Japanese Lung Cancer
MEK1 and AKT2 Mutations in Japanese Lung Cancer
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DOI：
10.1097/jto.0b013e3181d35236
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发表时间：
2010-05
影响因子：
20.4
通讯作者：
H. Sasaki;Y. Hikosaka;O. Kawano;S. Moriyama;M. Yano;Y. Fujii
中科院分区：
文献类型：
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作者：
H. Sasaki;Y. Hikosaka;O. Kawano;S. Moriyama;M. Yano;Y. Fujii
背景资料：近年来，为确定表皮生长因子受体（EGFR）信号通路基因的潜在体细胞突变，在非小细胞肺癌中发现了MEK 1基因第2外显子突变和AKT 2（v-akt murine thymoma viral oncogene homologue 2）基因激酶结构域突变。方法：对280例经手术治疗的非小细胞肺癌患者的MEK 1基因突变和273例AKT 2基因突变进行研究。采用直接测序法分析MEK 1外显子2突变和AKT 2激酶结构域突变的存在与否。结果如下：280例肺癌患者中有1例（0.4%）发现MEK 1突变（K57 K），仅1例（0.4%）发现AKT 2突变（R371 H）。MEK 1突变与EGFR、K-ras和B-raf激酶结构域突变无关。AKT 2突变与EGFR和PIK 3CA突变并存。结论：这项研究表明，AKT 2激酶结构域突变和MEK 1外显子2突变发生在一小部分日本肺癌。
Background: Recently, to identify potential somatic mutations in genes of epidermal growth factor receptor (EGFR) signaling pathway, MEK1 gene mutation at exon 2 and mutation of the AKT2 (v-akt murine thymoma viral oncogene homologue 2) gene at kinase domain have been reported in non-small cell lung cancer. Methods: We investigated the MEK1 mutation (n = 280) and AKT2 mutation (n = 273) in surgically treated non-small cell lung cancer cases. The presence or absence of MEK1 mutation (exon 2) and AKT2 mutation at kinase domain was analyzed by direct sequences. Results: MEK1 mutation (K57K) was found from 1 of 280 patients with lung cancer (0.4%) and detected only one case (0.4%) of AKT2 mutation (R371H) in our cohort. MEK1 mutation was exclusive with EGFR, K-ras, and B-raf mutations at kinase domain. However, AKT2 mutation was coexisted with EGFR and PIK3CA mutations. Conclusion: This study demonstrated that mutation in the kinase domain of AKT2 and MEK1 exon 2 mutation occurred in a small fraction of Japanese lung cancers.