Accumulation and cross-linkage of β-1,3/1,6-glucan lead to loss of basal stipe cell wall extensibility in mushroom Coprinopsis cinerea
Accumulation and cross-linkage of β-1,3/1,6-glucan lead to loss of basal stipe cell wall extensibility in mushroom Coprinopsis cinerea
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β-1,3/1,6-葡聚糖的积累和交联导致蘑菇灰霉病菌基部柄细胞壁延伸性丧失
DOI：
10.1016/j.carbpol.2021.117743
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发表时间：
2021-02-05
影响因子：
11.2
通讯作者：
Yuan, Sheng
中科院分区：
文献类型：
--
作者：
Li, Maomao;Bi, Jingjing;Yuan, Sheng
蘑菇成熟的基柄失去了壁的延伸性。我们发现来自灰霉的内切-13-1，3葡聚糖酶ENG可以通过预处理恢复成熟的基柄壁伸长性，这样几丁质酶ChiIII就可以诱导ENG处理的基柄壁伸长。ENG处理从基柄壁上释放出葡萄糖、层二糖和3-O-D-龙胆二糖-D-葡萄糖，与ENG消化的13-1，6-支链13-1，3-葡聚糖的产物一致。分析了内切-13-1，3-葡聚糖酶ENG和外切-13-1，3-葡聚糖酶EXG对基柄和顶端柄细胞壁产物的结构、数量和比例(13-1，3-糖苷键与13-1，6-糖苷键)的不同影响，以及对基柄细胞壁延伸性和细胞壁超微结构的影响。结果表明，随着壁厚的成熟，13-1，6-支链的13-1，3-葡聚糖的积累和交联度较高，导致基柄区域的壁延展性比顶端柄细胞壁的延伸性丧失。
The mature basal stipe of mushroom Coprinopsis cinerea loses wall extensibility. We found that an endo-13-1,3glucanase ENG from C. cinerea could restore mature basal stipe wall extensibility via pretreatment such that the ENG-pretreated basal stipe walls could be induced to extend by chitinase ChiIII. ENG pretreatment released glucose, laminaribiose, and 3-O-D-gentiobiose-D-glucose from the basal stipe walls, consistent with ENG-digested products of 13-1,6-branched 13-1,3-glucan. Different effects of endo-13-1,3-glucanase ENG and exo-13-1,3-glucanase EXG pretreatment on the structure, amount and ratio (13-1,3-glucoside bonds to 13-1,6-glucoside bonds) of products from the basal stipe and the apical stipe cell walls, respectively, and on the cell wall extensibility and the cell wall ultra-architecture of the basal stipes were analyzed. All results demonstrate that the more accumulation and cross-linkage of 13-1,6-branched 13-1,3-glucan with wall maturation lead to loss of wall extensibility of the basal stipe regions compared to the apical stipe cell walls.