Formation of Multiphase Complex Coacervates and Partitioning of Biomolecules within them
Formation of Multiphase Complex Coacervates and Partitioning of Biomolecules within them
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DOI：
10.1021/acs.biomac.9b01354
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发表时间：
2020-02-01
影响因子：
6.2
通讯作者：
Keating, Christine D.
中科院分区：
文献类型：
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作者：
Mountain, Gregory A.;Keating, Christine D.
生物系统使用液-液相分离来定位大分子和过程。细胞内凝析油允许多个不同的液体隔间的性质以及它们的共存对相组成和溶质分配的影响还不是很清楚。在这里，我们通过基于离子配对的复合凝聚产生两个和三个共存的富含大分子的液体隔室，所述混合物包含两个或三个聚阴离子以及一个、两个或三个聚阳离子。虽然在一些体系中，聚电解质的加入顺序对于实现液相共存很重要，但对另一些体系则不是，这表明所观察到的多相液滴形态在能量上是有利的。聚电解质分布在所有凝聚相上，这取决于它们之间的相对相互作用，这反过来又影响寡核苷酸和寡肽溶质的分配。这些结果表明，通过多相复杂凝聚体系固有的聚电解质共享，可以容易地生成多相凝聚物，并能够调整其分配性能。
Biological systems employ liquid-liquid phase separation to localize macromolecules and processes. The properties of intracellular condensates that allow for multiple, distinct liquid compartments and the impact of their coexistence on phase composition and solute partitioning are not well understood. Here, we generate two and three coexisting macromolecule-rich liquid compartments by complex coacervation based on ion pairing in mixtures that contain two or three polyanions together with one, two, or three polycations. While in some systems polyelectrolyte order-of-addition was important to achieve coexisting liquid phases, for others it was not, suggesting that the observed multiphase droplet morphologies are energetically favorable. Polyelectrolytes were distributed across all coacervate phases, depending on the relative interactions between them, which in turn impacted partitioning of oligonucleotide and oligopeptide solutes. These results show the ease of generating multiphase coacervates and the ability to tune their partitioning properties via the polyelectrolyte sharing inherent to multiphase complex coacervate systems.