Effects of LncRNA Lnc-LIF-AS on cell proliferation, migration and invasion in a human cervical cancer cell line
Effects of LncRNA Lnc-LIF-AS on cell proliferation, migration and invasion in a human cervical cancer cell line
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LncRNA Lnc-LIF-AS对人宫颈癌细胞系增殖、迁移和侵袭的影响
DOI：
10.1016/j.cyto.2019.05.004
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发表时间：
2019-08-01
期刊：
影响因子：
3.8
通讯作者：
Zhou, Ying
中科院分区：
文献类型：
--
作者：
Song, Weiguo;Wang, Juan;Zhou, Ying
本研究探讨了LncRNA Lnc-LIF-AS对人宫颈癌（HCC）细胞系SiHa增殖、迁移和侵袭的影响。SiHa细胞Lnc-LIF-AS的表达在4种人宫颈癌细胞系中最低（SiHa，ME-180，C-33 A和HeLa）转染，并分为SiHa/con（转染pMIGRI）细胞组，SiHa/Lnc-LIF-AS（转染pMIGRI-Lnc-LIF-AS）细胞组和SiHa/Lnc-LIF-AS-DN（转染pMIGRI-Lnc-LIF-AS-DN，其中与LIF mRNA重叠的序列被删除）细胞组。过表达Lnc-LIF-AS可促进SiHa和ME-180细胞的增殖、集落形成、侵袭和迁移。Lnc-LIF-AS的低表达抑制HeLa细胞的增殖、集落形成、侵袭和迁移。SiHa/Lnc-LIF-AS细胞组细胞周期主要停滞在S期，Lnc-LIF-AS过表达对SiHa细胞凋亡无影响。过表达Lnc-LIF-AS可促进SiHa/con细胞分泌LIF，SiHa/Lnc-LIF-AS细胞培养上清可促进SiHa/con细胞增殖。STAT 3抑制剂可抑制SiHa/Lnc-LIF-AS细胞的增殖。Lnc-LIF-AS在宫颈癌组织中的表达水平高于正常宫颈组织，且Lnc-LIF-AS的表达水平与LIF水平呈正相关。SiHa/con和SiHa/Lnc-LIF-AS-DN细胞组在细胞增殖、细胞迁移和细胞侵袭方面无显著差异。Lnc-LIF-AS的过表达可促进宫颈癌细胞的增殖、迁移和侵袭，该lncRNA的核心功能域位于与LIF mRNA的3 '-UTR碱基序列重叠的区域。
This study explored the effect of LncRNA Lnc-LIF-AS on cell proliferation, migration and invasion in the human cervical cancer (HCC) cell line SiHa. SiHa cells had the lowest expression of Lnc-LIF-AS in the 4 human cervical cancer cell lines (SiHa, ME-180, C-33A and HeLa) and were transfected and divided into the SiHa/con (transfected with pMIGRI) cell group, SiHa/Lnc-LIF-AS (transfected with pMIGRI-Lnc-LIF-AS) cell group, and SiHa/Lnc-LIF-AS-DN (transfected with pMIGRI-Lnc-LIF-AS-DN, in which the sequences overlapping with LIF mRNA was deleted) cell group. Overexpression of Lnc-LIF-AS could promote the proliferation, colony formation, invasion and migration in SiHa and ME-180 cells. And the low expression of Lnc-LIF-AS suppress the proliferation, colony formation invasion and migration in HeLa cells when the Lnc-LIF-AS expression has been suppressed. In the SiHa/Lnc-LIF-AS cells group, the cell cycle was mainly halted in the S phase and overexpression of Lnc-LIF-AS had no effect on the apoptosis of SiHa cells. Overexpression of Lnc-LIF-AS could promote the secretion of LIF in SiHa cells, and the supernatant from SiHa/Lnc-LIF-AS cells could promote cell proliferation in the SiHa/con cells. The STAT3 inhibitor could inhibit cell proliferation in the SiHa/Lnc-LIF-AS cells. The expression level of Lnc-LIF-AS in cervical cancer tissues was higher than that in normal tissues and the expression level of Lnc-LIF-AS was positively correlated with the level of LIF. In the SiHa/con and SiHa/Lnc-LIF-AS-DN cell groups, there were no significant differences in cell proliferation, cell migration and cell invasion. The overexpression of Lnc-LIF-AS can promote cell proliferation, migration and invasion in cervical cancer cells, and the core function domain of this lncRNA was located in the overlapping a 3'-UTR base sequence of LIF mRNA.