Using pressure-driven flow systems to evaluate laser speckle contrast imaging.
Using pressure-driven flow systems to evaluate laser speckle contrast imaging.
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DOI：
10.1117/1.jbo.28.3.036003
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发表时间：
2023-03
影响因子：
3.5
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中科院分区：
文献类型：
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微流体流模型研究通常用于表征激光散斑对比成像 (LSCI) 仪器的性能。流量控制系统的选择对于测试过程中流量的可靠生成至关重要。最近大多数使用微流体的 LSCI 研究都使用注射泵进行流量控制。我们量化了注射泵和压力调节流量系统的流量产生的不确定性。然后，我们使用压力调节流量系统在一定流速范围内评估了 LSCI 和多次曝光散斑成像 (MESI) 的性能。在使用嵌入式流量传感器的微流体装置中进行阶梯式流量剖面实验期间，对注射泵和压力调节流量系统进行了评估。计算与每个流量系统相关的不确定性并用于确定仪器测试的可靠性。然后使用压力调节流量系统来表征阶梯式流量剖面实验期间 LSCI 和 MESI 的相对性能，同时使用嵌入式流量传感器作为参考。与注射泵相比，压力调节流量系统产生更加稳定和可重复的流量输出。在测试的流速范围内，注射泵的扩展不确定性比压力调节流量系统的扩展不确定性高 8 倍。使用压力调节流量系统，MESI 在所有流速下都优于单次曝光 LSCI，并且密切反映流量传感器测量结果，平均误差分别为 和 。在通过微流体研究评估流量测量技术的性能时，应使用压力调节流量系统代替注射泵。 MESI 在各种流速范围内提供比传统 LSCI 更准确的相对流量测量。
Microfluidic flow phantom studies are commonly used for characterizing the performance of laser speckle contrast imaging (LSCI) instruments. The selection of the flow control system is critical for the reliable generation of flow during testing. The majority of recent LSCI studies using microfluidics used syringe pumps for flow control. We quantified the uncertainty in flow generation for a syringe pump and a pressure-regulated flow system. We then assessed the performance of both LSCI and multi-exposure speckle imaging (MESI) using the pressure-regulated flow system across a range of flow speeds. The syringe pump and pressure-regulated flow systems were evaluated during stepped flow profile experiments in a microfluidic device using an inline flow sensor. The uncertainty associated with each flow system was calculated and used to determine the reliability for instrument testing. The pressure-regulated flow system was then used to characterize the relative performance of LSCI and MESI during stepped flow profile experiments while using the inline flow sensor as reference. The pressure-regulated flow system produced much more stable and reproducible flow outputs compared to the syringe pump. The expanded uncertainty for the syringe pump was 8 to higher than that of the pressure-regulated flow system across the tested flow speeds. Using the pressure-regulated flow system, MESI outperformed single-exposure LSCI at all flow speeds and closely mirrored the flow sensor measurements, with average errors of and , respectively. Pressure-regulated flow systems should be used instead of syringe pumps when assessing the performance of flow measurement techniques with microfluidic studies. MESI offers more accurate relative flow measurements than traditional LSCI across a wide range of flow speeds.