THE APPLICATION OF A HOMOGENEOUS HALF-SPACE MODEL IN THE ANALYSIS OF ENDOTHELIAL-CELL MICROPIPETTE MEASUREMENTS
THE APPLICATION OF A HOMOGENEOUS HALF-SPACE MODEL IN THE ANALYSIS OF ENDOTHELIAL-CELL MICROPIPETTE MEASUREMENTS
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DOI：
10.1115/1.3108430
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发表时间：
1988-08-01
影响因子：
1.7
通讯作者：
WHEELER, LT
中科院分区：
文献类型：
--
作者：
THERET, DP;LEVESQUE, MJ;WHEELER, LT
实验研究表明，已经暴露于剪切应力的内皮细胞在脱离后保持扁平和细长的形状。它们的机械性能，这是使用微量移液管实验研究，受到水平以及剪切应力的持续时间。在本文中，我们分析了这些机械性能的两个数学模型的帮助下建议的微量吸管技术和这些细胞特有的几何形状，在他们的分离后曝光状态。这两种模型的不同之处在于它们对细胞和微量移液器之间的接触区的处理。的主要结果是一个有效的杨氏模量的细胞，这是用来与微管数据结合，以确定一个有效的杨氏模量牛内皮细胞的表达式，并讨论这个模量的依赖性后，暴露于剪切应力。
Experimental studies have shown that endothelial cells which have been exposed to shear stress maintain a flattened and elongated shape after detachment. Their mechanical properties, which are studied using the micropipette experiments, are influenced by the level as well as the duration of the shear stress. In the present paper, we analyze these mechanical properties with the aid of two mathematical models suggested by the micropipette technique and by the geometry peculiar to these cells in their detached post-exposure state. The two models differ in their treatment of the contact zone between the cell and the micropipette. The main results are expressions for an effective Young''s modulus for the cells, which are used in conjunction with the micropipette data to determine an effective Young''s modulus for bovine endothelial cells, and to discuss the dependence of this modulus upon exposure to shear stress.