PPARγ2 Gene Pro12Ala and PPARα Gene Leu162Val Single Nucleotide Polymorphisms Interact with Dietary Intake of Fat in Determination of Plasma Lipid Concentrations
PPARγ2 Gene Pro12Ala and PPARα Gene Leu162Val Single Nucleotide Polymorphisms Interact with Dietary Intake of Fat in Determination of Plasma Lipid Concentrations
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DOI：
10.1159/000336362
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发表时间：
2011-01-01
影响因子：
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通讯作者：
O'Dell, Sandra D.
中科院分区：
文献类型：
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作者：
AlSaleh, Aseel;Frost, Gary S.;O'Dell, Sandra D.
背景/目标：过氧化物酶体增殖物激活受体（PPARs）是脂质代谢的转录调节因子，由不饱和脂肪酸激活。我们研究了47.5%的代谢综合征受试者中，PPAR γ 2基因PPARG Pro 12 Ala（rs 1801282）和PPAR α基因PPARA Leu 162 Val（rs 1800206）基因型与膳食脂肪酸摄入量对血脂浓度的独立和交互作用。方法：RISCK研究是一项平行设计、随机对照试验。在基线时，在4周的高饱和脂肪酸饮食后，并在三个平行的24周的干预与参考（高饱和脂肪酸），高单不饱和脂肪酸和低脂饮食后，定量血脂。对466例受试者进行单核苷酸多态性基因分型。结果如下：基线时，PPARG Ala 12等位基因与血浆总胆固醇（n = 378; p = 0.04）、LDL胆固醇（p = 0.05）和apoB（p = 0.05）升高相关，校正年龄、性别和种族后。基线时，PPARA Leu 162 Val x PPARG Pro 12 Ala基因型相互作用对血脂浓度无显著影响。饮食干预后，基因-基因相互作用显著影响LDL胆固醇（p = 0.0002）和小密度LDL（p = 0.005）的比例调整后的LDL。结论：PPARG Pro 12 Ala和PPARA Leu 162 Val基因型之间的相互作用可能影响高单不饱和脂肪酸饮食后血浆LDL胆固醇浓度和小密度LDL的比例。版权所有（C）2012 S. Karger AG，巴塞尔
Background/Aims: The peroxisome proliferator-activated receptors (PPARs) are transcriptional regulators of lipid metabolism, activated by unsaturated fatty acids. We investigated independent and interactive effects of PPAR gamma 2 gene PPARG Pro12Ala (rs1801282) and PPAR alpha gene PPARA Leu162Val (rs1800206) genotypes with dietary intake of fatty acids on concentrations of plasma lipids in subjects of whom 47.5% had metabolic syndrome. Methods: The RISCK study is a parallel design, randomised controlled trial. Plasma lipids were quantified at baseline after a 4-week high saturated fatty acids diet and after three parallel 24-week interventions with reference (high saturated fatty acids), high monounsaturated fatty acids and low-fat diets. Single nucleotide polymorphisms were genotyped in 466 subjects. Results: At baseline, the PPARG Ala12 allele was associated with increased plasma total cholesterol (n = 378; p = 0.04), LDL cholesterol (p = 0.05) and apoB (p = 0.05) after adjustment for age, gender and ethnicity. At baseline, PPARA Leu162Val x PPARG Pro12Ala genotype interaction did not significantly influence plasma lipid concentrations. After dietary intervention, gene-gene interaction significantly influenced LDL cholesterol (p = 0.0002) and small dense LDL as a proportion of LDL (p = 0.005) after adjustments. Conclusions: Interaction between PPARG Pro12Ala and PPARA Leu162Val genotypes may influence plasma LDL cholesterol concentration and the proportion as small dense LDL after a high monounsaturated fatty acids diet. Copyright (C) 2012 S. Karger AG, Basel