Targeting colloidal particulates to thoracic lymph nodes
Targeting colloidal particulates to thoracic lymph nodes
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DOI：
10.1016/j.lungcan.2005.11.006
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发表时间：
2006-03-01
期刊：
影响因子：
5.3
通讯作者：
Johnston, MR
中科院分区：
文献类型：
--
作者：
Liu, J;Wong, HL;Johnston, MR
背景：胸腔淋巴管和淋巴结可受癌症等疾病的影响。微米或纳米颗粒被用作治疗剂的载体。方法：将不同微米级和纳米级的木炭、聚苯乙烯和聚丙交酯-乙交酯微纳米粒分别注入大鼠胸膜腔内，研究微粒从胸腔到胸腔淋巴结的淋巴分布。实验动物模型包括健康大鼠、肺切除后大鼠和原位肺癌大鼠，以模拟相关的临床情景和病理生理学。结果：我们发现：(1)所研究的颗粒在胸腔内被区域胸腔淋巴系统清除；(2)淋巴摄取早在注射后3h就开始出现，并且主要通过壁层胸膜；(3)在所有动物模型中都一致地发现了颗粒向区域淋巴结的转运；(4)直径在0.7~2微米的粒子分布最佳。结论：胸膜腔内注射碰撞粒子可触及局部胸腔内的淋巴结和淋巴结。(C)2005爱思唯尔爱尔兰有限公司。保留所有权利。
Background: Thoracic lymphatics and lymph nodes can be affected by cancer and other diseases. Micro- or nanoparticulates are used as carriers for therapeutic agents. We investigated the Lymphatic distribution after intrapleural administration of three different particulates.Methods: Various micro- and nanoparticles of charcoal, polystyrene and poly(lactide-co-glycolide) were administered into the pleural space of rats to study the lymphatic distribution of particles from the pleural cavity to the thoracic lymph nodes. Experimental animal models included healthy rats, rats following pneumonectomy and rats bearing orthotopic lung cancer to mimic relevant clinical scenarios and pathophysiology. Macroscopic examination, tight microscopy, fluorescence microscopy complemented by transmission electron microscopy analysis of the lymphatic tissue allowed precise mapping and detection of the particulates in the thoracic lymphatic system.Results: We found that (1) the particles studied are cleared by the regional thoracic lymphatic system when placed in the pleural space; (2) the lymphatic uptake occurred as early as 3 h after injection and is primarily through the parietal pleura; (3) the transport of particles to regional lymph nodes was consistently found in all three animal models; (4) particles of 0.7-2 mu m in size give the best lymphatic distribution.Conclusions: Regional thoracic lmphatics and lymph nodes can be accessed by collidal particles injected into the pleural space. (c) 2005 Elsevier Ireland Ltd. All rights reserved.