Functional understanding of the diverse exon-intron structures of human GPCR genes.
Functional understanding of the diverse exon-intron structures of human GPCR genes.
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DOI：
10.1142/s0219720013500194
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发表时间：
2014-02
影响因子：
1
通讯作者：
Xu Y
中科院分区：
文献类型：
--
作者：
Hammond DA;Olman V;Xu Y
GPCR基因具有多种外显子-内含子结构，尽管它们的蛋白质在结构上都是同源的。我们已经检查了所有人类GPCR基因与至少两个功能蛋白质亚型，共199，旨在获得什么可能有助于GPCR基因的外显子-内含子结构的巨大多样性的理解。这199个基因共有808种已知的蛋白质剪接异构体，具有实验验证的功能。我们的分析显示，在199个基因中的总共1，613个中的1，301个（80.6%）相邻外显子-外显子对在808种蛋白质剪接异构体中的至少一种中具有恰好一个外显子跳过或中间的内含子保留。假设观察到的剪接异构体与外显子-内含子结构无关，则该观察结果具有统计学显著性p值2.051762* e-09。我们对这一观察结果的解释是，GPCR基因的外显子边界不是随机确定的;相反，它们可能是为了功能目的而选择的，以促进特定的选择性剪接。
The GPCR genes have a variety of exon-intron structures even though their proteins are all structurally homologous. We have examined all human GPCR genes with at least two functional protein isoforms, totaling 199, aiming to gain an understanding of what may have contributed to the large diversity of the exon-intron structures of the GPCR genes. The 199 genes have a total of 808 known protein splicing isoforms with experimentally verified functions. Our analysis reveals that 1,301 (80.6%) adjacent exon-exon pairs out of the total of 1,613 in the 199 genes have either exactly one exon skipped or the intron in-between retained in at least one of the 808 protein splicing isoforms. This observation has a statistical significance p-value of 2.051762* e−09, assuming that the observed splicing isoforms are independent of the exon-intron structures. Our interpretation of this observation is that the exon boundaries of the GPCR genes are not randomly determined; instead they may be selected to facilitate specific alternative splicing for functional purposes.