THE PENICILLIN GENE-CLUSTER IS AMPLIFIED IN TANDEM REPEATS LINKED BY CONSERVED HEXANUCLEOTIDE SEQUENCES
THE PENICILLIN GENE-CLUSTER IS AMPLIFIED IN TANDEM REPEATS LINKED BY CONSERVED HEXANUCLEOTIDE SEQUENCES
复制标题
DOI：
10.1073/pnas.92.13.6200
复制
发表时间：
1995-06-20
影响因子：
11.1
通讯作者：
MARTIN, JF
中科院分区：
文献类型：
--
作者：
FIERRO, F;BARREDO, JL;MARTIN, JF
产黄青霉AS-P-78的青霉素生物合成基因(pbcAB、pBC、Pende)位于106.5 kb的DNA区域，由保守的TTTACA序列串联重复扩增(5或6个拷贝)，野生型菌株Prysgenum NRRL1951和青霉ATCC9478(Fleming‘s分离物)含有106，5 kb区域的单一拷贝。该区域与在AS-P-78株的串联重复序列之间发现的相同的TTTACA六核苷酸相邻。青霉素高产菌株P.chrysgenum E1含有一个57.9kb的DNA片段，由相同的六核苷酸或其反向互补的TGTAAA序列相连，缺失突变株P.chrysgenum npe10缺失了57.9kb的DNA片段，与在E1中扩增的DNA片段完全一致。保守的六核苷酸序列在缺失位置重组，扩增发生在单个染色体(I号染色体)内，串联重复和缺失似乎是通过突变诱导的保守六核苷酸序列的定点重组而发生的。
The penicillin biosynthetic genes (pbcAB, pcbC, penDE) of Penicillium chrysogenum AS-P-78 were located in a 106.5-kb DNA region that is amplified in tandem repeats (five or six copies) linked by conserved TTTACA sequences, The wild-type strains P. chrysogenum NRRL 1951 and Penicillium notatum ATCC 9478 (Fleming's isolate) contain a single copy of the 106,5-kb region. This region was bordered by the same TTTACA hexanucleotide found between tandem repeats in strain AS-P-78. A penicillin overproducer strain, P. chrysogenum E1, contains a large number of copies in tandem of a 57.9-kb DNA fragment, linked by the same hexanucleotide or its reverse complementary TGTAAA sequence, The deletion mutant P. chrysogenum npe10 showed a deletion of 57.9 kb that corresponds exactly to the DNA fragment that is amplified in E1. The conserved hexanucleotide sequence was reconstituted at the deletion site, The amplification has occurred within a single chromosome (chromosome I), The tandem reiteration and deletion appear to arise by mutation-induced site-specific recombination at the conserved hexanucleotide sequences.