Immune responses induced by different genotypes of the disease-specific protein of Rice stripe virus in the vector insect
Immune responses induced by different genotypes of the disease-specific protein of Rice stripe virus in the vector insect
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水稻条纹病毒病特异性蛋白不同基因型在媒介昆虫体内诱导的免疫反应
DOI：
10.1016/j.virol.2018.11.011
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发表时间：
2019
期刊：
影响因子：
3.7
通讯作者：
Cui Feng
中科院分区：
文献类型：
--
作者：
Zhao Wan;Wang Qianshuo;Xu Zhongtian;Liu Renyi;Cui Feng
持久性植物病毒在寄主植物和媒介昆虫之间传播，可能导致病毒种群的遗传分化。分析了水稻条纹病毒（Rice stripe virus，RSV）在褐飞虱和水稻中的单核苷酸多态性（single nucleotide polymorphism，SNPs）。导致RSV疾病特异性蛋白（SP）中非同义取代的两个SNP产生了三种基因型，即，GG、AA和GA。GG型主要存在于RSV在稻飞虱体内的感染早期，在病毒传播过程中逐渐被其他两种基因型所取代。这两个SNP不影响SP与水稻PsbP或RSV外壳蛋白的相互作用。在模式识别分子和免疫应答效应器通路中，SP GG基因型诱导的免疫应答比其他两种基因型更强。这些结果表明RSV在媒介昆虫和寄主植物之间的循环过程中存在种群变异。
Persistent plant viruses circulate between host plants and vector insects, possibly leading to the genetic divergence in viral populations. We analyzed the single nucleotide polymorphisms (SNPs) ofRice stripe virus(RSV) when it incubated in the small brown planthopper and rice. Two SNPs, which lead to nonsynonymous substitutions in the disease-specific protein (SP) of RSV, produced three genotypes, i.e., GG, AA and GA. The GG type mainly existed in the early infection period of RSV in the planthoppers and was gradually substituted by the other two genotypes during viral transmission. The two SNPs did not affect the interactions of SP with rice PsbP or with RSV coat protein. The GG genotype of SP induced stronger immune responses than those of the other two genotypes in the pattern recognition molecule and immune-responsive effector pathways. These findings demonstrated the population variations of RSV during the circulation between the vector insect and host plant.