Nociceptive responses to thermal and mechanical stimulations in awake pigs
Nociceptive responses to thermal and mechanical stimulations in awake pigs
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DOI：
10.1002/j.1532-2149.2012.00228.x
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发表时间：
2013-05-01
影响因子：
3.6
通讯作者：
Herskin, M. S.
中科院分区：
文献类型：
--
作者：
Di Giminiani, P.;Petersen, L. J.;Herskin, M. S.
背景猪皮肤与人皮肤具有高度的同源性，并且猪最近被用作皮肤疼痛模型。然而，在这种新的体内皮肤疼痛模型的全部潜力可以实现之前，与在大物种中管理清醒动物研究相关的几个方法学方面需要进一步检查。这份手稿描述了皮肤伤害感受的猪模型的初步发展，并侧重于感觉模态，身体大小和刺激部位的解剖位置之间的相互作用。方法采用热刺激（CO2激光）和机械刺激（压力测量装置）对不同体重（30和60 kg）的猪的侧腹和后肢进行热刺激和机械刺激。记录中位反应潜伏期和行为反应类型。结果小型猪对热刺激和机械刺激的痛阈明显低于大型猪。在两个解剖位置的刺激引起了非常不同的行为反应，与不同水平的灵敏度之间的侧翼和后腿。此外，小动物在单次刺激之间表现出较低水平的个体变异性。结论我们的数据表明，这种实验方法可能是有价值的，用于研究，重点是猪皮肤伤害性感受。
Background Porcine skin exhibits a high degree of homology to human skin, and the pig has recently been used as a cutaneous pain model. However, before the full potential of this novel in vivo cutaneous pain model can be achieved, several methodological aspects related to the management of awake animal studies in a large species require further examination. This manuscript describes the initial development of a porcine model of cutaneous nociception and focuses on interactions between the sensory modality, body size and the anatomical location of the stimulation site. Methods Pigs of different body sizes (30 and 60kg) were exposed to thermal (CO2 laser) and mechanical (pressure application measurement device) stimulations to the flank and the hind legs in a balanced order. The median response latency and the type of behavioural response were recorded. Results Small pigs exhibited significantly lower pain thresholds (shorter latency to response) than large pigs to thermal and mechanical stimulations. Stimulations at the two anatomical locations elicited very distinct sets of behavioural responses, with different levels of sensitivity between the flank and the hind legs. Furthermore, small animals exhibited lower levels of individual variability between single stimulations. Conclusions Our data indicate that this experimental approach may be valuable for use in studies that focus on porcine cutaneous nociception.