Pre-inoculation of urinary catheters with Escherichia coli 83972 inhibits catheter colonization by Enterococcus faecalis
Pre-inoculation of urinary catheters with Escherichia coli 83972 inhibits catheter colonization by Enterococcus faecalis
复制标题
DOI：
10.1016/s0022-5347(05)65471-7
复制
发表时间：
2002-01-01
影响因子：
6.6
通讯作者：
Thornby, JI
中科院分区：
文献类型：
--
作者：
Trautner, BW;Darouiche, RO;Thornby, JI
目的：研究了大肠杆菌83972的预先存在的涂层减少粪肠球菌210在导管中定植的能力。肠球菌是一种常见的泌尿系病原体，在留置导尿管的患者，因为它被选为这些trials。材料和方法：每个实验测试3个生长条件。组1或E. coli + Enterococcus导管暴露于E. coli 83972 24 h后再转化Enterococcus 30 min。组2或E. coli单独的导管在E. coli中培养24小时，然后在无菌肉汤中培养30分钟。第3组或单独的肠球菌导管不进行与E.在与肠球菌孵育30分钟之前，将所有导管在无菌人尿液中孵育24小时。用盐水洗涤导管并切成5.1 cm。片段对每个节段进行超声处理，并将超声处理液稀释并铺板。取5个节段的结果的平均值，并重复7次实验。coli 83972减少了E.粪菌210多10倍。肠球菌单独导管的中位数为9.7 × 10（5）肠球菌/cin，而E.大肠杆菌加肠球菌导管的平均值为0.38 × 10（5）个肠球菌/cm。结论：留置尿管前感染大肠埃希菌的患者，其尿流率明显高于留置尿管后感染大肠埃希菌的患者（p = 0.016）。大肠杆菌83972显着阻碍导管定植肠球菌：这些有前途的体外结果提示这种特殊的应用细菌干扰的临床研究。
Purpose: The capacity of a preexisting coating of Escherichia coli 83972 to reduce catheter colonization by Enterococcus faecalis 210 was investigated. Enterococcus was chosen for these trials since it is a common urinary pathogen in patients with an indwelling urinary catheter.Materials and Methods: Each experiment tested 3 growth conditions. Group 1 or E. coli plus Enterococcus catheters were exposed to E. coli 83972 for 24 hours and then to Enterococcus for 30 minutes. Group 2 or E. coli alone catheters were incubated in E. coli for 24 hours and then in sterile broth for 30 minutes. Group 3 or Enterococcus alone catheters did not undergo the initial incubation with E. coli before the 30-minute incubation with Enterococcus: All catheters were then incubated in sterile human urine for 24 hours. Catheters were washed with saline and cut into 5, 1 cm. segments. Each segment was sonicated and the sonication fluid was diluted and plated. The results of each of the 5 segments were averaged and the set of experiments was repeated 7 times.Results: A preexisting coating of E. coli 83972 reduced catheter colonization by E. faecalis 210 more than 10-fold. Enterococcus alone catheters had a median of 9.7 X 10(5) enterococci per cin., whereas E. coli plus Enterococcus catheters had a median of 0.38 X 10(5) enterococci per cm. (p = 0.016).Conclusions: Pre-inoculating urinary catheters with E. coli 83972 significantly impedes catheter colonization by Enterococcus: These promising in vitro results prompt the clinical investigation of this particular application of bacterial interference.