The human granulocyte nucleus: Unusual nuclear envelope and heterochromatin composition
The human granulocyte nucleus: Unusual nuclear envelope and heterochromatin composition
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DOI：
10.1016/j.ejcb.2008.02.007
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发表时间：
2008-05-01
影响因子：
6.6
通讯作者：
Olins, Donald E.
中科院分区：
文献类型：
--
作者：
Olins, Ada L.;Zwerger, Monika;Olins, Donald E.
人类血液中的粒细胞（中性粒细胞）适合于发现和破坏感染因子。人类嗜中性粒细胞的核具有分段的外观，由三个或四个裂片的线性或分支阵列组成。足够水平的核纤层蛋白B受体（LBR）是分叶核分化所必需的。核被膜其他成分的水平也可能对核形状的确定很重要。在本研究中，免疫染色和免疫印迹程序探讨了核膜和异染色质的各种成分的水平，比较新鲜分离的人中性粒细胞与粒细胞形式的HL-60细胞，组织培养模型系统。与粒细胞HL-60细胞相比，血液中性粒细胞核膜含有低至可忽略量的LBR、核纤层蛋白A/C、B1和B2、LAP 2 β和emerin。令人惊讶的是，与粒细胞HL-60细胞相比，“有丝分裂”染色体标记物H3（S10）phos在中性粒细胞核中升高。此外，中性粒细胞核对甲醇固定似乎比粒细胞HL-60细胞更脆弱。因此，人类中性粒细胞核似乎是高度特化的，具有核分裂稳定蛋白的缺乏。因此，中性粒细胞核似乎具有很强的延展性，支持通过紧密的组织间隙快速迁移。(C)2008年Elsevier GmbH。All rights reserved.
The human blood granulocyte (neutrophil) is adapted to find and destroy infectious agents. The nucleus of the human neutrophil has a segmented appearance, consisting of a linear or branched array of three or four lobes. Adequate levels of lamin B receptor (LBR) are necessary for differentiation of the lobulated nucleus. The levels of other components of the nuclear envelope may also be important for nuclear shape determination. In the present study, immunostaining and immunoblotting procedures explored the levels of various components of the nuclear envelope and heterochromatin, comparing freshly isolated human neutrophils with granulocytic forms of HL-60 cells, a tissue culture model system. In comparison to granulocytic HL-60 cells, blood neutrophil nuclear envelopes contain low-to-negligible amounts of LBR, lamins A/C, B1 and B2, LAP2 beta and emerin. Surprisingly, a "mitotic" chromosome marker, H3(S10)phos, is elevated in neutrophil nuclei, compared to granulocytic HL-60 cells. Furthermore, neutrophil nuclei appear to be more fragile to methanol fixation, than observed with granulocytic HL-60 cells. Thus, the human neutrophil nucleus appears to be highly specialized, possessing a paucity of nuclear envelope-stabilizing proteins. In consequence, the neutrophil nucleus appears to be very malleable, supporting rapid migration through tight tissue spaces. (C) 2008 Elsevier GmbH. All rights reserved.