Candida albicans Amphotericin B-Tolerant Persister Formation is Closely Related to Surface Adhesion.
Candida albicans Amphotericin B-Tolerant Persister Formation is Closely Related to Surface Adhesion.
复制标题
白色念珠菌两性霉素 B 耐受持久性的形成与表面粘附密切相关。
DOI：
10.1007/s11046-015-9894-1
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发表时间：
2016
期刊：
影响因子：
5.5
通讯作者：
Qi Qingguo
中科院分区：
文献类型：
--
作者：
Sun Jing;Li Zhigang;Chu Haoyue;Guo Jing;Jiang Guangshui;Qi Qingguo
迄今为止，仅在生物膜环境中观察到白色念珠菌持续存在;触发持续存在形成的生物膜元件仍然未知。在本研究中，我们试图进一步阐明C. albicanspersisters和生物膜形成的早期阶段，特别是表面粘附阶段。3C调查了白念珠菌菌株的持留菌形成情况。我们测试了C。在粘附和生物膜形成过程中的不同时间点，白色孢子粉的动态形成。基于对阿替霉素B处理后细胞活力的评估和集落形成单位测定来确定持续存在细胞的数量。没有一种植物的培养物中含有持久性细菌。紧接着粘附C.白念珠菌细胞附着于生物膜表面后，出现持留细胞，持留细胞比例在约2 h达到高峰，为0.2- 0.69%。随着生物膜的成熟，持留菌的比例下降，到24 h仅为0.01- 0.02%，而持留菌的数量保持稳定，没有显著变化。在不存在预涂覆的附着表面的情况下，未检测到持久性。Persisters也不存在于生物膜中，所述生物膜在阿替霉素B处理之前被刮擦以破坏表面粘附。这些结果表明C.白念珠菌耐药持留菌主要产生于表面粘附阶段，表面粘附是白念珠菌产生和维持的必要条件。albicanspersisters.
Candida albicanspersisters have so far been observed only in biofilm environment; the biofilm element(s) that trigger(s) persister formation are still unknown. In this study, we tried to further elucidate the possible relationship betweenC. albicanspersisters and the early phases of biofilm formation, especially the surface adhesion phase. ThreeC. albicansstrains were surveyed for the formation of persisters. We testedC. albicanspersister formation dynamically at different time points during the process of adhesion and biofilm formation. The number of persister cells was determined based on an assessment of cell viability after amphotericin B treatment and colony-forming unit assay. None of the planktonic cultures contained persisters. Immediately following adhesion ofC. albicanscells to the surface, persister cells emerged and the proportion of persisters reached a peak of 0.2–0.69 % in approximately 2-h biofilm. As the biofilm matured, the proportion of persisters decreased and was only 0.01–0.02 % by 24 h, while the number of persisters remained stable with no significant change. Persisters were not detected in the absence of an attachment surface which was pre-coated. Persisters were also absent in biofilms that were scraped to disrupt surface adhesion prior to amphotericin B treatment. These results indicate thatC. albicansantifungal-tolerant persisters are produced mainly in surface adhesion phase and surface adhesion is required for the emergence and maintenance ofC. albicanspersisters.