Puncture-mediated gene transfer to the skin
Puncture-mediated gene transfer to the skin
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DOI：
10.1089/hum.1996.7.8-893
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发表时间：
1996-05-20
影响因子：
4.2
通讯作者：
Carbone, DP
中科院分区：
文献类型：
--
作者：
Ciernik, IF;Krayenbuhl, BH;Carbone, DP
简单而有效的基因转移到皮肤将促进许多局部和全身的基因治疗应用。在这里，我们报告了一种新的方法，允许安装和质粒DNA的表达没有颗粒载体进入皮肤的基因治疗的目的。具有恒定高频振荡的细金属针束的装置允许刺穿皮肤并导致DNA转移到小鼠中的皮肤相关细胞和报告基因的表达。此外，刺穿介导的编码单个T细胞表位的表达载体的滴注诱导特异性细胞免疫应答。如果可以优化这种方法，穿刺介导的基因转移可能用于以体细胞基因治疗为目的的质粒DNA治疗大的身体区域。
Simple and efficient gene transfer to the skin would facilitate many local and systemic gene therapy applications. Here we report a novel approach that allows installation and expression of plasmid DNA without a particulate carrier into the skin for the purposes of gene therapy. A device with a constantly high-frequency oscillating bundle of fine metal needles allows puncturing of the skin and leads to DNA transfer to skin-associated cells and expression of reporter genes in mice, Furthermore, puncture-mediated instillation of an expression vector coding for a single T cell epitope induces specific cellular immune responses, If this approach could be optimized, puncture-mediated gene transfer might be useful for the treatment of large body areas with plasmid DNA for the purpose of somatic gene therapy.