Wavelength-based localized surface plasmon resonance optical fiber biosensor
Wavelength-based localized surface plasmon resonance optical fiber biosensor
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DOI：
10.1016/j.snb.2013.02.052
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发表时间：
2013-05-01
影响因子：
8.4
通讯作者：
Grattan, Kenneth T. V.
中科院分区：
文献类型：
--
作者：
Cao, Jie;Tu, Minh Hiue;Grattan, Kenneth T. V.
两种类型的局域表面等离子体共振（LSPR）为基础的光纤生物传感器，使用金纳米球（GNSs）和金纳米棒（GNRs）已开发和成功的性能特性进行评估和交叉比较，在这项工作中。基于从这些类型的生物传感器的每一个的优化获得的结果和作者在其他地方报道的，在这项工作中特别选择了直径为60 nm的GNS和纵横比为4.1的GNR用于制造两个代表性的传感器探针，目的是创建一个高度灵敏的和基于波长的LSPR传感器，以克服其他基于强度的传感器所产生的限制。为了开发有效的LSPR生物传感器，在用人IgG官能化之前，将GNS和GRN分别固定在光纤的无包层表面上，以产生用于检测不同浓度的抗人IgG的装置。从所进行的测试中获得的实验结果表明，基于GNS和GNRs的LSPR传感器对折射率变化的灵敏度分别为914和601 nm/RIU;然而，作为生物传感器，它们对于检测抗人IgG表现出相同的检测限1.6 nM。(c)2013爱思唯尔有限公司版权所有。
Two types of localized surface plasmon resonance (LSPR)-based optical fiber biosensors using gold nanospheres (GNSs) and gold nanorods (GNRs) have been developed and their performance characteristics evaluated and cross-compared successfully in this work. Based on the results obtained from the optimization of each of these types of biosensor and reported by the authors elsewhere, GNSs with a diameter of 60 nm and GNRs with an aspect ratio of 4.1 were specifically chosen in this work for the fabrication of two representative sensor probes, with an aim to create a highly sensitive and wavelength-based LSPR sensor to overcome the limitations arising from other intensity-based sensors. In order to develop effective LSPR biosensors, both GNSs and GRNs respectively were immobilized on an unclad surface of an optical fiber, prior to the functionalization with human IgG in order to create a device for the detection of anti-human IgG, at different concentrations. The experimental results obtained from tests carried out show that the sensitivities of GNSs and GNRs-based LSPR sensors to refractive index variation are 914 and 601 nm/RIU respectively; however as biosensors they have demonstrated the same detection limit of 1.6 nM for the detection of anti-human IgG. (c) 2013 Elsevier B.V. All rights reserved.