Reduction of heavy metal (Pb2+) biosorption in zebrafish model using alginic acid purified from Ecklonia cava and two of its synthetic derivatives
Reduction of heavy metal (Pb2+) biosorption in zebrafish model using alginic acid purified from Ecklonia cava and two of its synthetic derivatives
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DOI：
10.1016/j.ijbiomac.2017.08.027
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发表时间：
2018-01-01
影响因子：
8.2
通讯作者：
Jeon, You-Jin
中科院分区：
文献类型：
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作者：
Fernando, I. P. Shanura;Sanjeewa, K. K. Asanka;Jeon, You-Jin
重金属污染因其生物吸附、生物蓄积和毒性而成为导致严重环境和健康问题的主要问题。本研究旨在评估从棕色海藻 Ecklonia cava 中提取的海藻酸 (AA) 及其两种衍生物的重金属螯合能力，该两种衍生物通过 2 度羟基 (OM) 的部分氧化和单体单元 (CM) 的部分羧化来减少斑马鱼 (Danio rerio) 模式中的重金属生物吸附。使用 ICP-OES 对金属离子进行定量，并通过 FTIR 和 XRD 分析对生物聚合物进行表征。所有研究的生物聚合物均显示出螯合 Pb2+、Cu2+、Cd2+、As3+ 和 Ag+ 的潜在能力。吸附容量大小顺序为CAA>OAA>AA。所有生物聚合物均表现出对 Pb2+ 的相对较高的螯合作用。 AA、OAA 和 CM 可以有效降低斑马鱼胚胎中 Pb2+ 诱导的毒性和 Pb2+ 应激诱导的 ROS 产生。此外，它们还可以减少成年斑马鱼对 Pb2+ 的生物吸附，从而导致生物蓄积。由于从大肠菌中纯化的海藻酸及其衍生物可以用作海藻衍生的生物聚合物来净化重金属污染的水，并作为膳食补充剂来减少生物体中的重金属生物吸附。 (C) 2017 Elsevier B.V. 保留所有权利。
Heavy metal contamination has become a major problem that causes severe environmental and health issues due to their biosorption, bioaccumulation, and toxicity. This study was designed to evaluate heavy metal chelating abilities of alginic acid (AA) extracted from the brown seaweed Ecklonia cava and two of its derivatives prepared by the partial oxidation of the 2 degrees OH groups (OM) and partial carboxylation of the monomeric units (CM) upon reducing the heavy metal biosorption in zebrafish (Danio rerio) modal. Metal ions were quantified using ICP-OES and biopolymers were characterized by FTIR and XRD analysis. All investigated biopolymers indicated potential ability for chelating Pb2+, Cu2+, Cd2+, As3+, and Ag+. The sorption capacities were in the order of CAA>OAA>AA. All biopolymers indicated a comparatively higher chelation towards Pb2+. AA, OAA, and CM could effectively reduce Pb2+ induced toxicity and Pb2+ stress induced ROS production in zebrafish embryos. Besides, they could reduce the biosorption of Pb2+ in adult zebrafish which could lead to bioaccumulation. Since alginic acid purified from E. cava and its derivatives could be utilized as seaweed derived biopolymers to purify heavy metals contaminated water and as a dietary supplement to reduce heavy metal biosorption in organisms. (C) 2017 Elsevier B.V. All rights reserved.