Light Dose and Time Dependency of Photodynamic Cell Membrane Damage
Light Dose and Time Dependency of Photodynamic Cell Membrane Damage
复制标题
光动力细胞膜损伤的光剂量和时间依赖性
DOI：
10.1111/j.1751-1097.1998.tb02539.x
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发表时间：
1998
影响因子：
3.3
通讯作者：
I. Karube
中科院分区：
文献类型：
--
作者：
T. Saito;N. Hartell;H. Muguruma;Shuichro Hotta;S. Sasaki;Masao Ito;I. Karube
我们用电生理学方法研究了光动力细胞膜损伤的光剂量和时间依赖性。这项研究通过精确地给药光剂量来控制细胞膜的损伤程度。光敏剂为5′，5″‐bis(aminomethyl)‐2，2′：5′，2″‐terthiophene二盐酸盐(BAT)。使用激光共聚焦扫描显微镜提供快速光激活(<；1 S)，并使用标准膜片钳技术监测随后的膜损伤。在49μM BAT存在下，低于0.94J/cm~2的光强导致可逆去极化(20 MV)，在3 S光照范围内，电阻减小(10%)。较高的光照强度(1.57J/cm~2)可导致细胞膜电位和细胞膜电阻在8 S光照范围内完全且不可逆地丧失。在抗氧化剂Trolox-C的存在下，在BAT存在的情况下，通过光照诱导细胞死亡所需的光阈值剂量增加。
We have investigated the light dose and time dependency of photodynamic cell membrane damage using electrophysiological methods. This study controls the level of cell membrane damage by precisely administration of the light dose. The photosensitizer used was 5′,5″‐bis(aminomethyl)‐2,2′:5′,2″‐terthiophene dihydrochloride (BAT). A confocal laser scanning microscope was used to provide rapid light activation (<1 s) and the subsequent membrane damage was monitored using standard patch clamp techniques. In the presence of 49 μM BAT, light levels less than 0.94 J/cm2 led to a reversible depolarization (20 mV) and reduction of resistance (10%) within 3 s of illumination. Higher intensities of illumination (1.57 J/cm2) caused a complete and irreversible loss of membrane potential and cell membrane resistance within 8 s of illumination. The threshold dose of light required to induce cell death by illumination in the presence of BAT was increased in the presence of the antioxidant Trolox‐C.
光诱导细胞质膜损伤的动力学。
影响因子：
3.4
作者：
Thorpe,WP;Toner,M;Ezzell,RM;Tompkins,RG;Yarmush,ML
通讯作者：
Yarmush,ML
短暂暴露于活性氧后心脏钾电流改变的进展。
影响因子：
5
作者：
Tarr,M;Arriaga,E;Valenzeno,D
通讯作者：
Valenzeno,D