Tube radial distribution chromatography system developed by combining commercially available HPLC system and open-tubular capillary tube as separation column.
Tube radial distribution chromatography system developed by combining commercially available HPLC system and open-tubular capillary tube as separation column.
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将市售高效液相色谱系统与开管毛细管作为分离柱相结合而开发的管径向分布色谱系统。
DOI：
10.1016/j.talanta.2018.02.046
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发表时间：
2018
期刊：
影响因子：
6.1
通讯作者：
K. Tsukagoshi
中科院分区：
文献类型：
--
作者：
Kento Yamada;Hyo Kan;K. Tsukagoshi
已经报道了基于开管毛细管中的管径向分布流或环状流的管径向分布色谱，其中环状流通过相分离多相流产生。色谱系统需要一些特定的仪器和处理方法来处理毛细管中的微流体流动。在这项研究中，我们开发了一种新的设置为管径向分布色谱相结合的市售HPLC系统与开放的管状毛细管（内径为100 µm）作为分离柱，而不是传统的填充柱。用进样阀进样分析物溶液（2 μL体积），并将水/乙腈/乙酸乙酯（3：8：2体积比）的三元溶液作为洗脱液以8.6 μL min−1的流速输送至毛细管中。该色谱系统，即配备开管毛细管的高效液相色谱系统，能够成功地分离模型分析物，1-萘酚，1-萘磺酸，2，6-萘二磺酸，基线分离。在径向分布色谱系统中，环状流中的内相和外相分别作为移动的和准固定相。将实验获得的分析物的洗脱时间与使用其内相和外相的容量因子和各相的线性流速计算的相应理论值进行比较。
Tube radial distribution chromatography based on tube radial distribution flow, or annular flow, in an open-tubular capillary has been reported, where the annular flow is created through phase separation multiphase flow. The chromatographic system requires some specific instruments and treatments for microfluidic flow in the capillary tube. In this study, we developed a new set-up for tube radial distribution chromatography by combining a commercially available HPLC system with an open-tubular capillary tube (with an inner diameter of 100 µm) as a separation column instead of a conventional packed column. The analyte solution was injected with an injection valve (2 μL volume) and a ternary solution of water/acetonitrile/ethyl acetate (3:8:2 vol ratio) was delivered as the eluent to the capillary tube at a flow rate of 8.6 μL min−1. The chromatographic system, that is, the HPLC system equipped with the open-tubular capillary tube, could successfully separate the model analytes, 1-naphthol, 1-naphthalenesulfonic acid, and 2,6-naphthalenedisulfonic acid, with base-line separation. The inner and outer phases in the annular flow worked as the mobile and pseudo-stationary phases, respectively, in the tube radial distribution chromatography system. The experimentally obtained elution times of the analytes were compared with their corresponding theoretical values calculated using their capacity factors for the inner and outer phases and the linear flow velocities of the respective phases.
层流条件下基于载体溶剂管径向分布支持毛细管色谱的荧光观察
影响因子：
4.2
作者：
Naoya Jinno;Mari Murakami;Kiyoshi Mizohata;Masahiko Hashimoto;Kazuhiko Tsukagoshi
通讯作者：
Kazuhiko Tsukagoshi
溶质分布与大口径毛细管中的层流相结合：没有化学或物理场可以分离什么？
DOI：
--
发表时间：
2005
期刊：
Analytical Sciences 21
影响因子：
--
作者：
Makoto Harada;Tomoo Kido;Takasi Masudo;Tetsuo Okada
通讯作者：
Tetsuo Okada