Development of a chemiluminescent optical fiber immunosensor to detect Streptococcus pneumoniae antipolysaccharide antibodies
Development of a chemiluminescent optical fiber immunosensor to detect Streptococcus pneumoniae antipolysaccharide antibodies
复制标题
DOI：
10.1385/abab:89:2-3:117
复制
发表时间：
2000-11-01
影响因子：
3
通讯作者：
Dagan, R
中科院分区：
文献类型：
--
作者：
Marks, RS;Margalit, A;Dagan, R
开发了一种基于化学发光的光纤免疫传感器用于检测抗肺炎球菌抗体。这是通过开发一种不同的化学方法实现的，该方法利用3 - 氨丙基三甲氧基硅烷和三聚氰氯将肺炎球菌细胞壁多糖结合到光纤尖端，并提高了光电检测系统的灵敏度。光纤检测到的抗肺炎球菌抗体的最低滴度为1 : 819200稀释度。标准酶联免疫吸附测定的最低相应值为1 : 98415稀释度。结论是，光纤免疫传感器系统是一种检测抗肺炎球菌抗体的准确且灵敏的方法，并且可能是监测唾液和尿液等样本中抗体的合适工具。
A chemiluminescent-based optical fiber immunosensor was developed for the detection of antipneumococcal antibodies. This was accomplished by developing a different chemical procedure utilizing 3-aminopropyl trimethoxysilane and cyanuric chloride to conjugate pneumococcal cell wall polysaccharides to the optical fiber tips, and by improving the sensitivity of the photodetection system. The lowest titer of antipneumococcal antibodies detected by the optical fiber was at a 1:819,200 dilution. The lowest corresponding value by standard enzyme-linked immunosorbent assay was at a 1:98,415 dilution. It was concluded that the optical immunosensor system is an accurate and sensitive method to detect antipneumococcal antibodies and may be an adequate tool to monitor antibodies in specimens such as saliva and urine.