Signal signature of aboveground-induced resistance upon belowground herbivory in maize
Signal signature of aboveground-induced resistance upon belowground herbivory in maize
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DOI：
10.1111/j.1365-313x.2009.03868.x
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发表时间：
2009-07-01
期刊：
影响因子：
7.2
通讯作者：
Ton, Jurriaan
中科院分区：
文献类型：
--
作者：
Erb, Matthias;Flors, Victor;Ton, Jurriaan
植物在受到胁迫时激活局部和系统的防御机制.这种先天免疫反应部分受植物激素调节，并涉及防御代谢物的积累。虽然当地的防御反应，草食动物的研究，了解较少的影响，根草食动物对拍摄防御。在这里，我们研究了地下侵染西方玉米根虫玉米根萤叶甲对玉米地上抗性的影响。地下食草动物D. v. virgifera诱导的地上部对通性草食动物Spodoptera littoralis和坏死型病原体Setosphaeria turcica的抗性。此外，D.在玉米根上接种玉米根后，玉米根上的2，4-二羟基-7-甲氧基-1，4-苯并恶嗪-3-酮（DIMBOA）含量增加，并在随后的S.海滨鱼为了深入了解这种地下和地上防御相互作用背后的信号网络，我们编制了一组32个防御相关基因，这些基因可用作转录标记系统来检测不同防御反应途径的活动。地下攻击D。在芽中引发ABA诱导的转录模式。防御激素的定量显示，在根和芽感染后，由D。v. virgifera和S. littoralis分别。另一方面，阿坝在地下受到D.玉米根此外，D.玉米根减少了地上部分的含水量，而去除同样数量的根系生物量则没有影响。本研究表明，根食性D. v. virgifera具体改变了玉米地上防御状态，并表明阿坝在介导这种相互作用的信号网络中发挥作用。
P>Plants activate local and systemic defence mechanisms upon exposure to stress. This innate immune response is partially regulated by plant hormones, and involves the accumulation of defensive metabolites. Although local defence reactions to herbivores are well studied, less is known about the impact of root herbivory on shoot defence. Here, we examined the effects of belowground infestation by the western corn rootworm Diabrotica virgifera virgifera on aboveground resistance in maize. Belowground herbivory by D. v. virgifera induced aboveground resistance against the generalist herbivore Spodoptera littoralis, and the necrotrophic pathogen Setosphaeria turcica. Furthermore, D. v. virgifera increased shoot levels of 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA), and primed the induction of chlorogenic acid upon subsequent infestation by S. littoralis. To gain insight into the signalling network behind this below- and aboveground defence interaction, we compiled a set of 32 defence-related genes, which can be used as transcriptional marker systems to detect activities of different hormone-response pathways. Belowground attack by D. v. virgifera triggered an ABA-inducible transcription pattern in the shoot. The quantification of defence hormones showed a local increase in the production of oxylipins after root and shoot infestation by D. v. virgifera and S. littoralis, respectively. On the other hand, ABA accumulated locally and systemically upon belowground attack by D. v. virgifera. Furthermore, D. v. virgifera reduced the aboveground water content, whereas the removal of similar quantities of root biomass had no effect. Our study shows that root herbivory by D. v. virgifera specifically alters the aboveground defence status of a maize, and suggests that ABA plays a role in the signalling network mediating this interaction.