Mechanisms of chemical mutagenesis and carcinogenesis: effects on DNA replication of methylation at the O6-guanine position of dGTP.
Mechanisms of chemical mutagenesis and carcinogenesis: effects on DNA replication of methylation at the O6-guanine position of dGTP.
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DOI：
10.1093/carcin/4.12.1591
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发表时间：
1983-12
期刊：
影响因子：
4.7
通讯作者：
D. Toorchen;M. D. Topal
中科院分区：
文献类型：
--
作者：
D. Toorchen;M. D. Topal
测定了“Klenow”大肠杆菌 pol I 体外 DNA 复制过程中 O6-甲基-dGTP 的掺入。发现 O6-甲基-dGTP 能够：(i) 掺入相反的 T 和 C 模板残基，对 T 的偏好超过 20 倍；(ii) 当掺入代替 dATP 时，除了富含嘧啶的生长链序列外，会阻止 DNA 合成。讨论了体内 DNA 生物合成过程中 O6-甲基-dGTP 掺入对于突变和致癌的重要性。
The incorporation of O6-methyl-dGTP during DNA replication in vitro by 'Klenow' E. coli pol I was determined. O6-Methyl-dGTP was found to: (i) incorporate opposite T and C template residues, with a greater than 20-fold preference for T, and (ii) arrest DNA synthesis when incorporated in place of dATP at all but pyrimidine-rich growing-strand sequences. The significance of O6-methyl-dGTP incorporation during DNA biosynthesis in vivo for mutagenesis and carcinogenesis is discussed.