Altered expression of piRNAs and their relation with clinicopathologic features of breast cancer
Altered expression of piRNAs and their relation with clinicopathologic features of breast cancer
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DOI：
10.1007/s12094-012-0966-0
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发表时间：
2013-07-01
影响因子：
3.4
通讯作者：
Zhang, X.
中科院分区：
文献类型：
--
作者：
Huang, G.;Hu, H.;Zhang, X.
PIWI相互作用RNA（piRNA）是内源性调节非编码RNA，其通常在动物物种的种系中表达。piRNA在肿瘤组织中的表达目前尚未见报道。为了研究piRNA在乳腺癌组织中的表达情况，对4例乳腺癌手术切除组织和4例配对的非肿瘤组织进行深度测序，筛选差异表达的piRNA。采用实时荧光定量RT-PCR（real-time reverse transcription-polymerase chain reaction，RT-PCR）技术检测50例乳腺癌患者皮尔纳的表达水平，并与临床病理特征进行相关性分析;通过深度测序，筛选出6条piRNA在4例乳腺癌组织中的表达差异（5条上调，1条下调）。其中，4个piRNA（piR-4987、piR-20365、piR-20485和piR-20582）通过实时RT-PCR证实在50个乳腺癌中上调（P < 0.001）。piR-4987表达上调与淋巴结阳性相关（P = 0.001），提示piRNA可能在乳腺癌中起一定作用，可作为肿瘤标志物。
PIWI interacting RNAs (piRNAs) are endogenous regulatory non-coding RNAs which are generally expressed in the germline across animal species. The expressions of piRNAs in tumor tissue were rarely reported until now. The aim of this study was to investigate the expression of piRNAs in breast cancer.Deep sequencing was carried out in four tumor and four matched non-tumor tissues from resected breast cancer cases to screen out differentially expressed piRNAs. Furthermore, the result was evaluated by real-time reverse transcription-polymerase chain reaction (RT-PCR) technology in 50 patients with breast cancer and the piRNA expression levels were correlated with clinicopathologic features.Through deep sequencing, six differentially expressed piRNAs were screened out (5 up- and 1 down-regulated) in the four tumor tissues compared with matched non-tumor tissues. Among them, 4 piRNAs (piR-4987, piR-20365, piR-20485 and piR-20582) were confirmed to be up-regulated by realtime RT-PCR in 50 breast cancer (P < 0.001). Moreover, up-regulated piR-4987 expression was associated with lymph node positivity (P = 0.001).PiRNAs might play a role in breast cancer and act as tumor markers.