Bismuth@US-tubes as a Potential Contrast Agent for X-ray Imaging Applications.
Bismuth@US-tubes as a Potential Contrast Agent for X-ray Imaging Applications.
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DOI：
10.1039/c3tb20742k
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发表时间：
2013-10-07
期刊：
影响因子：
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通讯作者：
Wilson LJ
中科院分区：
文献类型：
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作者：
Rivera EJ;Tran LA;Hernández-Rivera M;Yoon D;Mikos AG;Rusakova IA;Cheong BY;Cabreira-Hansen MD;Willerson JT;Perin EC;Wilson LJ
在超短(约50 nm)单壁碳纳米胶囊(US-管)中实现了BiOCl/Bi2O_3对铋的包覆。用高分辨电子显微镜(HR-TEM)、能量色散X射线光谱(EDS)、热重分析(TGA)、X射线光电子能谱(XPS)和拉曼光谱对所制备的铋管进行了表征。首次将BI@US管用于猪骨髓间充质干细胞(MSCs)的细胞内标记，以显示CT细胞成像中的高X射线对比度。相对较高的对比度是通过较低的铋负载量(2.66%的重量百分比)在US管中实现的，并且不会影响细胞的活力。在100千伏CT临床扫描仪中，标记了BiUS管的MSCs的X射线CT成像显示，与未标记的MSCs相比，对比增强几乎增加了两倍。在相同浓度下，BiUS管的CT信号增强效果是聚合物包裹的Bi2S3纳米粒子的500倍，是任何临床碘化造影剂(CA)的数倍。我们的发现表明，BiUS管可以作为一种潜在的新型X射线CT试剂用于干细胞标记和可能的活体示踪。
The encapsulation of bismuth as BiOCl/Bi2O3 within ultra-short (ca. 50 nm) single-walled carbon nanocapsules (US-tubes) has been achieved. The Bi@US-tubes have been characterized by high-resolution transmission electron microscopy (HR-TEM), energy-dispersive X-ray spectroscopy (EDS), thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS), and Raman spectroscopy. Bi@US-tubes have been used for intracellular labeling of pig bone marrow-derived mesenchymal stem cells (MSCs) to show high X-ray contrast in computed tomography (CT) cellular imaging for the first time. The relatively high contrast is achieved with low bismuth loading (2.66% by weight) within the US-tubes and without compromising cell viability. X-ray CT imaging of Bi@US-tubes-labeled MSCs showed a nearly two-fold increase in contrast enhancement when compared to unlabeled MSCs in a 100 kV CT clinical scanner. The CT signal enhancement from the Bi@US-tubes is 500 times greater than polymer-coated Bi2S3 nanoparticles and several-fold that of any clinical iodinated contrast agent (CA) at the same concentration. Our findings suggest that the Bi@US-tubes can be used as a potential new class of X-ray CT agent for stem cell labeling and possibly in vivo tracking.