Silver against Pseudomonas aeruginosa biofilms
Silver against Pseudomonas aeruginosa biofilms
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DOI：
10.1111/j.1600-0463.2007.apm_646.x
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发表时间：
2007-08-01
期刊：
影响因子：
2.8
通讯作者：
Givskov, Michael
中科院分区：
文献类型：
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作者：
Bjarnsholt, Thomas;Kirketerp-Moller, Klaus;Givskov, Michael
几个世纪以来，银因其抗菌性能而被公认。大多数关于银抗菌功效的研究，特别强调伤口愈合，都是在嗜酸性细菌上进行的。然而，我们最近的研究强烈表明，伤口的定植涉及细菌的增殖和生物膜生长模式。研究了银对铜绿假单胞菌（慢性感染伤口的主要病原体）的成熟体外生物膜的作用。结果表明，银对铜绿假单胞菌的成熟生物膜非常有效，但银浓度是重要的。浓度为5-10 IG/mL的银磺胺嘧啶根除生物膜，而较低浓度（1 IG/mL）则没有效果。消除细菌生物膜所需的银的杀菌浓度比用于消除嗜热细菌的杀菌浓度高10-100倍。这些观察结果强烈表明，目前可用的伤口敷料中的银浓度对于治疗慢性生物膜伤口来说太低。建议临床医生和所述伤口敷料的制造商考虑其是否治疗主要由生物膜形成细菌或寄生细菌定殖的伤口。
Silver has been recognized for its antimicrobial properties for centuries. Most studies on the antibacterial efficacy of silver, with particular emphasis on wound healing, have been performed on planktonic bacteria. Our recent studies, however, strongly suggest that colonization of wounds involves bacteria in both the planktonic and biofilm modes of growth. The action of silver on mature in vitro biofilms of Pseudomonas aeruginosa, a primary pathogen of chronic infected wounds, was investigated. The results show that silver is very effective against mature biofilms of P. aeruginosa, but that the silver concentration is important. A concentration of 5-10 ig/mL silver sulfadiazine eradicated the biofilm whereas a lower concentration (1 ig/mL) had no effect. The bactericidal concentration of silver required to eradicate the bacterial biofilm was 10-100 times higher than that used to eradicate planktonic bacteria. These observations strongly indicate that the concentration of silver in currently available wound dressings is much too low for treatment of chronic biofilm wounds. It is suggested that clinicians and manufacturers of the said wound dressings consider whether they are treating wounds primarily colonized either by biofilm-forming or planktonic bacteria.