A HUMAN TELOMERIC PROTEIN
A HUMAN TELOMERIC PROTEIN
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DOI：
10.1126/science.270.5242.1663
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发表时间：
1995-12-08
期刊：
影响因子：
56.9
通讯作者：
DELANGE, T
中科院分区：
文献类型：
--
作者：
CHONG, L;VANSTEENSEL, B;DELANGE, T
端粒是多功能元件，其保护染色体末端免于降解和端对端融合，防止DNA损伤检查点的激活，并通过端粒酶调节端粒DNA的维持。人类端粒的一个主要蛋白质成分已经被鉴定和克隆。该因子TRF含有一个Myb型DNA结合重复序列和一个氨基末端酸性结构域。免疫荧光标记显示，TRF特异性地与端粒DNA共定位在人类间期细胞中，并位于染色体末端在中期。TRF沿着端粒TTAGGG重复序列的存在表明人类端粒形成了一种特殊的核蛋白复合物。
Telomeres are multifunctional elements that shield chromosome ends from degradation and end-to-end fusions, prevent activation of DNA damage checkpoints, and modulate the maintenance of telomeric DNA by telomerase. A major protein component of human telomeres has been identified and cloned. This factor, TRF, contains one Myb-type DNA-binding repeat and an amino-terminal acidic domain. Immunofluorescent labeling shows that TRF specifically colocalizes with telomeric DNA in human interphase cells and is located at chromosome ends during metaphase. The presence of TRF along the telomeric TTAGGG repeat army demonstrates that human telomeres form a specialized nucleoprotein complex.