EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice.
EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice.
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DOI：
10.7554/elife.59759
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发表时间：
2021-05-11
期刊：
影响因子：
7.7
通讯作者：
Sugiyama F
中科院分区：
文献类型：
--
作者：
Osawa Y;Murata K;Usui M;Kuba Y;Le HT;Mikami N;Nakagawa T;Daitoku Y;Kato K;Shawki HH;Ikeda Y;Kuno A;Morimoto K;Tanimoto Y;Dinh TTH;Yagami KI;Ema M;Yoshida S;Takahashi S;Mizuno S;Sugiyama F
雄性生殖细胞必须在每个分化阶段采用正确的形态，才能正确地发生精子。精原细胞通过自身的迁移来调节其分化状态。随着合胞体的形成，雄性生殖细胞分化成熟，未能形成合适的合胞体，导致精母细胞停滞。然而，雄性生殖细胞形态调控的详细分子机制尚不清楚。在这里，我们发现EXOC1是胞囊复合体的一员，在小鼠精原细胞和精母细胞合胞的伪足形成过程中起着重要的作用。EXOC1通过失活Rho家族的小GTP酶rac1参与精原细胞伪足的形成，并与SNARE蛋白STX2和SNAP23一起作用于精母细胞合胞体。由于EXOC1已知可与多种细胞形态发生因子结合，因此本研究有望成为发现多种雄性生殖细胞形态调控因子的起点。
The male germ cells must adopt the correct morphology at each differentiation stage for proper spermatogenesis. The spermatogonia regulates its differentiation state by its own migration. The male germ cells differentiate and mature with the formation of syncytia, failure of forming the appropriate syncytia results in the arrest at the spermatocyte stage. However, the detailed molecular mechanisms of male germ cell morphological regulation are unknown. Here, we found that EXOC1, a member of the Exocyst complex, is important for the pseudopod formation of spermatogonia and spermatocyte syncytia in mice. EXOC1 contributes to the pseudopod formation of spermatogonia by inactivating the Rho family small GTPase Rac1 and also functions in the spermatocyte syncytia with the SNARE proteins STX2 and SNAP23. Since EXOC1 is known to bind to several cell morphogenesis factors, this study is expected to be the starting point for the discovery of many morphological regulators of male germ cells.