The eyelid margin - A transitional zone for 2 epithelial phenotypes
The eyelid margin - A transitional zone for 2 epithelial phenotypes
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DOI：
10.1001/archopht.125.4.523
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发表时间：
2007-04-01
影响因子：
--
通讯作者：
Beuerman, Roger W.
中科院分区：
文献类型：
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作者：
Liu, Shaohui;Li, Jing;Beuerman, Roger W.
目的：目的：探讨灵长类眼睑边缘上皮的分化特征。方法：采用免疫组织化学方法检测3只成年食蟹猴眼睑组织中细胞角蛋白（CKs）CK 1/10、CK 4、CK 14、CK 5/8和CK 19、外皮蛋白、连接蛋白43、丝聚蛋白和M受体亚型（m1-m5）的表达。细胞角蛋白1/10、CK 4、CK 19、丝聚蛋白和连接蛋白43的表达在粘膜皮肤连接部（MCJ）的上皮中各不相同。在MCJ，CK 4阳性细胞重叠的基础CK 14表达细胞。睑板腺管和MCJ显示CK 14阳性细胞层。细胞角蛋白5/8表达于表皮和结膜的基底层，而外皮蛋白阳性细胞位于表面。M2在整个结膜中表达; M3在皮肤，结膜和睑板腺的肺泡上皮细胞的基底细胞中发现; M4在皮肤的基底层上表达。结论：眼睑边缘上皮由2个不同的上皮细胞亚群形成，具有特定但重叠的分布。睑板腺管或MCJ可能是一个网站的结膜祖细胞。临床相关性：这项研究提供了一个基础，了解眼睑病理条件，并最终为开发方法，用于细胞重建的眼睑边缘使用扩展的祖细胞。
Objective: To investigate the differentiation profile of the epithelium of the eyelid margin in the primate.Methods: The expression of cytokeratins (CKs) CK1/10, CK4, CK14, CK5/8, and CK19; involucrin; connexin 43; filaggrin; and muscarinic receptor subtypes (m1-m5) on eyelid tissues from adult Macaca fascicularis (n = 3) was studied by immunohistochemistry.Results: Cytokeratin 1/10, CK4, CK19, filaggrin, and connexin 43 expression varied across the epithelium of the mucocutaneous junction (MCJ). At the MCJ, CK4-positive cells overlapped basal CK14-expressing cells. The meibomian gland duct and the MCJ revealed layers of CK14-positive cells. Cytokeratin 5/8 was expressed in the basal layer of the epidermis and conjunctiva, while involucrin-positive cells were superficial. The m2 was expressed throughout the conjunctiva; m3 was found in the basal cells of the skin, conjunctiva, and alveolar epithelial cells of the meibomian glands; and m4 was expressed in the suprabasal layers of the skin.Conclusions: The eyelid margin epithelium is formed from 2 different epithelial cell subpopulations with specific but overlapping distributions. The meibomian gland duct or MCJ may be a site of conjunctival progenitor cells.Clinical Relevance: This study provides a basis for understanding eyelid pathological conditions and eventually for developing methods for cellular reconstruction of the eyelid margin using expanded progenitor cells.