Inhibitory Effects of Emodin, Thymol, and Astragalin on Leptospira interrogans-Induced Inflammatory Response in the Uterine and Endometrium Epithelial Cells of Mice
Inhibitory Effects of Emodin, Thymol, and Astragalin on Leptospira interrogans-Induced Inflammatory Response in the Uterine and Endometrium Epithelial Cells of Mice
复制标题
大黄素、百里酚和黄芪对问号钩端螺旋体诱导的小鼠子宫和子宫内膜上皮细胞炎症反应的抑制作用。
DOI：
10.1007/s10753-017-0513-9
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发表时间：
2017-04-01
期刊：
影响因子：
5.1
通讯作者：
Cao, Yongguo
中科院分区：
文献类型：
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作者：
Zhang, Wenlong;Lu, Xiaojie;Cao, Yongguo
钩端螺旋体病是一种全身性感染，可引起急性肾损伤、急性肝病、肌肉疼痛、血管炎、出血性疾病和生殖能力丧失等。为了减少钩端螺旋体引起的子宫炎症反应，我们在小鼠模型中评价了大黄素、百里酚和甘草素的抗炎作用。我们的研究结果表明，大黄素，麝香草酚和甘草酸治疗减轻子宫炎症引起的钩端螺旋体感染通过抑制促炎细胞因子的表达，并防止组织损伤。此外，我们使用原代子宫内膜上皮细胞显示，这些化学品的治疗抑制促炎细胞因子TNF-α，IL-1 β和IL-6的表达，采用酶联免疫吸附试验和定量聚合酶链反应。Western blot结果表明，这些化学品抑制p38，p65，细胞外信号调节激酶，和c-Jun N-末端激酶的磷酸化。这些结果表明，大黄素，麝香草酚和甘草酸治疗抑制炎症反应，通过调节NF-κ B和丝裂原活化蛋白激酶信号通路在钩端螺旋体感染的小鼠子宫和子宫内膜上皮细胞。
Leptospirosis is a systemic infection that causes, among others, acute kidney injury, acute liver disease, muscle pain, vasculitis, bleeding disorders, and reproductive loss. In an effort to reduce uterine inflammatory responses induced by Leptospira, we evaluated the anti-inflammation effects of emodin, thymol, and astragalin in a mouse model. Our results showed that treatment with emodin, thymol, and astragalin alleviated uterine inflammation induced by leptospira infection via suppression of pro-inflammatory cytokine expression and prevented tissue damage. Furthermore, we used primary endometrium epithelial cells to show that treatment with these chemicals inhibited the expression of proinflammatory cytokines TNF-alpha, IL-1 beta, and IL-6 using enzyme-linked immunosorbent assay and quantitative polymerase chain reaction. Western blot results showed that these chemicals suppressed the phosphorylation of p38, p65, extracellular signal-regulated kinase, and c-Jun N-terminal kinase. These results indicate that treatment with emodin, thymol, and astragalin suppressed inflammatory response by regulating NF-kappa B and mitogen-activated protein kinase signaling pathways in leptospira-infected uterine and endometrium epithelial cells of mice.