Quantitative 3D Micro-CT Imaging of Human Lung Tissue
Quantitative 3D Micro-CT Imaging of Human Lung Tissue
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DOI：
10.1055/s-0033-1350105
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发表时间：
2013-09-01
影响因子：
1.8
通讯作者：
Langheinrich, A. C.
中科院分区：
文献类型：
--
作者：
Kampschulte, M.;Schneider, C. R.;Langheinrich, A. C.
目的：评估micro-CT用于获得纤维化、肺气肿和非病变人肺标本中软组织、呼吸道和血管化变化的定量体积和形态学信息的可行性。材料和方法：对22例UIP型肺纤维化或10例小叶中心型肺气肿的尸检或肺活检标本进行显微CT扫描，并与对照组（n = 10）进行比较。22）。在血管内造影增强后进行成像，以评估血管容积分数。在固定通气肺后，使用锇进行组织对比增强，对软组织和空气分数进行定量。为了实现肺腺泡的无伪影三维重建，对肺气肿标本（n = 5）和非病变组织（n = 6）进行了基于同步加速器的显微CT扫描。Micro-CT成像的补充，组织学的示范可比findings.Results：定量分析显示，软组织分数的显着增加，相当于减少的空气分数在纤维化肺相比，对照组（p < 0.001）和显着减少的血管体积分数相比，对照组（p < 0.02）。与对照组相比，肺气肿样本的空气分数显著增加，软组织减少（p < 0.001）。肺腺泡的3D重建成功地在非病变组织，但未能在纤维化和肺气肿lungs.Conclusion：我们的研究结果表明，micro-CT的技术可行性，以评估定量和形态学数据从患病和非患病的人肺标本。
Purpose: To assess the feasibility of micro-CT for obtaining quantitative volumetric and morphologic information of changes in soft tissue, respiratory tracts and vascularization in fibrotic, emphysematous and non-diseased human lung specimens.Materials and Methods: Specimens from autopsy or lung explantation with lung fibrosis of UIP pattern (n = 22) or centrilobular emphysema (n = 10) were scanned by micro-CT and compared to controls (n = 22). Imaging was performed subsequent to intravascular contrast enhancement for the assessment of the vascular volume fraction. The soft tissue and air fraction were quantified after the fixation of ventilated lungs followed by tissue contrast enhancement using osmium. Aiming an artifact-free 3D reconstruction of lung acini, synchrotron-based micro-CT scans of specimens with emphysema (n = 5) and non-diseased tissue (n = 6) was performed. Micro-CT imaging was complemented by histology for the demonstration of comparable findings.Results: Quantitative analysis showed a significant increase of the soft tissue fraction, equivalent to a decrease of the air fraction in fibrotic lungs compared to controls (p < 0.001) and a significant reduction of the vascular volume fraction compared to controls (p < 0.02). Specimens with emphysema demonstrated a significant increase of the air fraction with a decrease in soft tissue compared to controls (p < 0.001). 3D reconstructions of lung acini worked successfully in non-diseased tissue but failed in fibrotic and emphysematous lungs.Conclusion: Our findings indicate micro-CT's technical feasibility to assess quantitative and morphological data from diseased and non-diseased human lung specimens.