A Cytological Analysis of Differentiation Without Cleavage in Cytochalasin B- and Colchicine-Treated Embryos of Chaetopterus pergamentaceus

A Cytological Analysis of Differentiation Without Cleavage in Cytochalasin B- and Colchicine-Treated Embryos of Chaetopterus pergamentaceus
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细胞松弛素 B 和秋水仙碱处理的毛毛蕨胚胎无裂解分化的细胞学分析

DOI:
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发表时间:
1981
期刊:
影响因子:
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通讯作者:
Yuan‐Hsu Kang
Yuan‐Hsu Kang
中科院分区:
--
文献类型:
--
作者:
W. Eckberg;Yuan‐Hsu Kang

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毛翅目卵在发育过程中经历特征性的卵质重排。在没有细胞分裂的情况下发生的卵质重排称为无卵裂的分化。用细胞松弛素B处理受精卵可以在没有细胞质分裂的情况下继续进行核分裂。未裂卵经细胞松弛素B处理后,其卵质重排与正常发育的卵质重排非常接近。秋水仙素处理,阻止有丝分裂,逮捕卵质运动的阶段相比,正常胚胎在第一次卵裂。这两种药物都不能消除透明质和内质之间的分离,即使秋水仙素阻止了后来的重排。因此,局部运动依赖于正常的微管功能,而不是微丝功能。局部物质的维持似乎并不完全依赖于这两种细胞器。
Chaetopterus eggs undergo characteristic ooplasmic rearrangements during development. Ooplasmic rearrangement in the absence of cell division is called differentiation without cleavage. Treatment of fertilized eggs with cytochalasin B allowed the continuation of nuclear divisions in the absence of cytoplasmic division. The ooplasmic rearrangements in uncleaved cytochalasin B-treated fertilized eggs closely paralleled those of normal development. Colchicine treatment, which blocks mitosis, arrested ooplasmic movements at a stage comparable to that of normal embryos at first cleavage. Neither drug eliminated the segregation between hyaloplasm and endoplasm, even though colchicine prevented the later rearrangements. Localizing movements are therefore dependent upon normal microtubule function, but not on microfilament function. The maintenance of localized materials does not seem to depend exclusively on either of these organelles.
细胞骨架抑制剂对 Chaetopterus pergamentaceus 细胞质定位的影响。
DOI: 10.1111/j.1432-0436.1981.tb01127.x
发表时间: 1981
期刊: Differentiation; research in biological diversity
影响因子: --
作者:
Eckberg,WR
通讯作者: Eckberg,WR