Using circular dichroism spectra to estimate protein secondary structure
Using circular dichroism spectra to estimate protein secondary structure
复制标题
DOI：
10.1038/nprot.2006.202
复制
发表时间：
2006-01-01
期刊：
影响因子：
14.8
通讯作者：
Greenfield, Norma J.
中科院分区：
文献类型：
--
作者：
Greenfield, Norma J.
圆二色性（CD）是快速测定重组蛋白或纯化蛋白二级结构和折叠特性的极好工具。蛋白质CD最广泛的应用是确定表达的纯化蛋白质是否折叠，或者突变是否影响其构象或稳定性。此外，它还可用于研究蛋白质相互作用。该协议详细说明了获得和解释CD数据的基本步骤，以及分析光谱以估计蛋白质二级结构组成的方法。CD的优点是可以对多个样品进行测量，
Circular dichroism (CD) is an excellent tool for rapid determination of the secondary structure and folding properties of proteins that have been obtained using recombinant techniques or purified from tissues. The most widely used applications of protein CD are to determine whether an expressed, purified protein is folded, or if a mutation affects its conformation or stability. In addition, it can be used to study protein interactions. This protocol details the basic steps of obtaining and interpreting CD data, and methods for analyzing spectra to estimate the secondary structural composition of proteins. CD has the advantage that measurements may be made on multiple samples containing