Catalytic promiscuity of halohydrin dehalogenase and its application in enantioselective epoxide ring opening
Catalytic promiscuity of halohydrin dehalogenase and its application in enantioselective epoxide ring opening
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DOI：
10.1002/cbic.200700734
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发表时间：
2008-05-05
期刊：
影响因子：
3.2
通讯作者：
Janssen, Dick B.
中科院分区：
文献类型：
--
作者：
Hasnaoui-Dijoux, Ghannia;Elenkov, Maja Majeric;Janssen, Dick B.
一般方法：在Bruker AV300(1H 300 MHz和13C 75.5 MHz)光谱仪上记录CDCl3的~1H和~(13)C核磁共振谱。化学位移(δ)是作为内部标准给出的，单位为ppm。在AEI MS-902仪器上记录了质谱(HRMS)。以氦气为载气，自动进样器进样(T=200oC)，在配有FID检测器(T=200oC)的Hewlett-Packard 6890系列色谱仪上进行了气相色谱分析。手性分析使用Chiraldex G-TA色谱柱(30 MX 0.25 mm x 0.25µm，ASTEC)和Chiraldex A-TA柱(30 MX 0.25 mm x 0.25µm，ASTEC)。材料：外消旋1，2-环氧丁烷(99%纯度)和对映体纯度(R)和(S)-1，2-环氧丁烷购自Aldrich Chemie。外消旋1，2-环氧丁烷在真空下蒸馏后用于酶促反应。外消旋丁烷-1，2-二醇、叠氮钠盐、氰化物、亚硝酸盐、氯化物、溴化物、碘化物、氰酸盐、硫氰酸盐和甲酸盐均从商业来源获得，无需进一步提纯即可使用。
General methods: 1H and 13C NMR spectra were recorded on a Bruker AV 300 (1H 300 MHz and 13C 75.5 MHz) spectrometer in CDCl3. Chemical shifts (δ) are given in ppm downfield from TMS as the internal standard. Mass spectra (HRMS) were recorded on an AEI MS-902 instrument. Gas chromatography (GC) was performed on Hewlett-Packard 6890 series gas chromatographs equipped with FID detectors (T= 200oC) by using He as the carrier gas and autosampler injection (T= 200oC). Chiral analyses were done using a Chiraldex G-TA column (30 mx 0.25 mm x 0.25 µm, Astec) and Chiraldex A-TA column (30 mx 0.25 mm x 0.25 µm, Astec).Materials: Racemic 1, 2-epoxybutane (99% purity) and enantiomerically pure (R) and (S)-1, 2-epoxybutane were purchased from Aldrich Chemie. Racemic 1, 2-epoxybutane was distilled under vacuum prior to utilization in enzymatic reactions. Racemic butane-1, 2-diol, sodium salts of azide, cyanide, nitrite, chloride, bromide, iodide, cyanate, thiocyanate and formate were obtained from commercial sources and used without further purification.