Identification and functional characterization of three type III polyketide synthases from Aquilaria sinensis calli.
Identification and functional characterization of three type III polyketide synthases from Aquilaria sinensis calli.
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DOI：
10.1016/j.bbrc.2017.03.159
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发表时间：
2017-05
影响因子：
3.1
通讯作者：
Xiao-hui Wang;Zhongxi Zhang;Xianjuan Dong;Yingying Feng;Xiao Liu;Bo-wen Gao;Jinling Wang;Le Zhang-L
中科院分区：
文献类型：
--
作者：
Xiao-hui Wang;Zhongxi Zhang;Xianjuan Dong;Yingying Feng;Xiao Liu;Bo-wen Gao;Jinling Wang;Le Zhang-L
III型聚酮合酶(PKS)在多种植物次生代谢产物的生物合成和植物适应环境胁迫方面发挥着重要作用。沉香(Aquilaria sinensis)是生产沉香的主要植物物种，对其PKS家族知之甚少。在这项研究中，AsCHS1和两个新的III型PKS，AsPKS1和AsPKS2，在A中被分离和表征。中华。序列比较和系统发育分析表明AsPKS1和AsPKS2属于与AsCHS1不同的non-CHS类群。重组AsPKS1和AsPKS2产生内酯型产物，表明它们的酶活性与AsCHS1不同。三个 PKS 基因在 A 中具有组织特异性模式。 此外，我们还检测了不同非生物胁迫和激素处理下愈伤组织中三个PKS基因的表达谱。盐胁迫诱导AsCHS1转录最显着，CdCl2处理诱导AsPKS1丰度最显着，甘露醇处理诱导AsPKS2表达最显着。此外，AsCHS1、AsPKS1和AsPKS2的表达在赤霉素(GA3)、茉莉酸甲酯(MeJA)或水杨酸(SA)处理下增强，而3个PKS基因在脱落酸(ABA)处理下早期表现出低转录水平。此外，三种GFP:PKS融合蛋白定位于本塞姆氏烟草细胞的细胞质和细胞壁中。这些结果表明三种 III 型 PKS 在聚酮化合物生物合成、植物对非生物胁迫的抗性和信号转导中的多功能作用。
Type III polyketide synthases (PKSs) play an important role in biosynthesis of various plant secondary metabolites and plant adaptation to environmental stresses.Aquilaria sinensis (A. sinensis)is the main plant species for production of agarwood, little is known about its PKS family. In this study,AsCHS1and two new type IIIPKSs,AsPKS1andAsPKS2, were isolated and characterized inA. sinensiscalli. The comparative sequence and phylogenetic analysis indicated that AsPKS1 and AsPKS2 belonged to non-CHS group different from AsCHS1. The recombinant AsPKS1 and AsPKS2 produced the lactone-type products, suggesting their different enzyme activities from AsCHS1. Three PKS genes had a tissues-specific pattern inA. sinensis.Moreover, we examined the expression profiles of threePKSgenes in calli under different abiotic stresses and hormone treatments.AsCHS1transcript was most significantly induced by salt stress,AsPKS1abundance was most remarkably enhanced by CdCl2treatment, whileAsPKS2expression was most significantly induced by mannitol treatment. Furthermore,AsCHS1, AsPKS1andAsPKS2expression was enhanced upon gibberellins (GA3), methyl jasmonate (MeJA), or salicylic acid (SA) treatment, while threePKSgenes displayed low transcript levels at the early stage under abscisic acid (ABA) treatment. In addition, three GFP:PKSs fusion proteins were localized in the cytoplasm and cell wall inNicotiana benthamianacells. These results indicated the multifunctional role of three type III PKSs in polyketide biosynthesis, plant resistance to abiotic stresses and signal transduction.