Angiogenin elevates the invasive potential of squamous cell lung carcinoma cells through epithelial‑mesenchymal transition.
Angiogenin elevates the invasive potential of squamous cell lung carcinoma cells through epithelial‑mesenchymal transition.
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DOI：
10.3892/or.2016.5107
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发表时间：
2016-11
期刊：
影响因子：
4.2
通讯作者：
Li Xu;Yan Yan-Yan;Xiang Xue;Chunguang Li;Zhiyun Xu;He-zhong Chen
中科院分区：
文献类型：
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作者：
Li Xu;Yan Yan-Yan;Xiang Xue;Chunguang Li;Zhiyun Xu;He-zhong Chen
肺鳞状细胞癌是最具侵袭性的癌症之一，其侵袭性部分是由于其固有的高转移率。此外，上皮-间质转化（epithelial-mesenchymal transition， EMT）过程似乎也参与了这些肿瘤的形成过程。此外，emt型细胞在鳞状细胞肺癌中具有许多与血管生成素（ANG）功能相同的生物学特性。我们采用免疫组化方法检测60例鳞状细胞肺癌组织中ANG、E-cadherin、vimentin、N-cadherin、β-catenin、TGF-β1的表达。采用Western blot法检测ANG和EMT标志物的蛋白表达水平。通过细胞计数试剂盒-8、划痕实验和Transwell侵袭室分析ANG对鳞状细胞肺癌细胞增殖、迁移和侵袭的影响，揭示ANG在鳞状细胞肺癌EMT过程中的作用。结果显示，ANG在鳞状细胞肺癌标本中异常表达，并与细胞系的分化密切相关。ANG的表达与鳞状细胞肺癌的转移和分期也有显著的相关性。此外，我们验证了ANG影响sk - mes1细胞中vimentin、E-cadherin、N-cadherin、β-catenin和TGF-β1的表达。最重要的是，ANG的过表达增强了SK-MES-1细胞的迁移和侵袭，而敲低则相反。在本研究中，我们发现ANG在鳞状细胞肺癌的EMT中发挥重要作用，可能是鳞状细胞肺癌有价值的治疗靶点。
Squamous cell carcinoma of the lung is one of the most aggressive cancers, and its aggressiveness is in part due to its intrinsic high rate of metastasis. Moreover, the process of epithelial-mesenchymal transition (EMT) appears to be involved in these neoplastic processes. Furthermore, EMT-type cells share many biological characteristics with the function of angiogenin (ANG) in squamous cell lung carcinoma. We conducted immunohistochemical analysis to detect the expression of ANG, E-cadherin, vimentin, N-cadherin, β-catenin and TGF-β1 in 60 cases of squamous cell lung carcinoma tissues. Western blot analysis was adopted to detect the protein expression levels of ANG and EMT markers. The effects of ANG on proliferation, migration and invasion of squamous cell lung carcinoma cells was analyzed by Cell Counting Kit-8, scratch assay and Transwell invasion chamber in order to reveal the role of ANG in the process of EMT in squamous cell lung carcinoma. The results revealed that ANG was aberrantly expressed in the squamous cell lung carcinoma specimens and was closely correlated with the differentiation of the cell lines. The expression of ANG was also significantly associated with metastasis and the stage of the squamous cell lung carcinoma cases. In addition, we validated that ANG influenced the expression of vimentin, E-cadherin, N-cadherin, β-catenin and TGF-β1 in SK-MES-1 cells. Most importantly, overexpression of ANG enhanced the migration and invasion of SK-MES-1 cells, while knockdown resulted in opposite effects. In the present study, we found that ANG plays an important role in EMT in squamous cell lung carcinoma and may be a valuable therapeutic target for squamous cell lung carcinoma.